Recombinant Mouse Semaphorin 4B Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10263-S4

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

Q62179.2

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Semaphorin 4B Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Semaphorin 4B protein
Mouse Semaphorin 4B
(Leu31-Glu703)
Accession # Q62179.2		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu31

Predicted Molecular Mass

102 kDa

SDS-PAGE

101-117 kDa, under reducing conditions

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant Mouse Semaphorin 4B Fc Chimera Protein, CF

Recombinant Mouse Semaphorin 4B Fc Chimera Protein SDS-PAGE

Recombinant Mouse Semaphorin 4B Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse Semaphorin 4B Fc Chimera (Catalog # 10263-S4) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 101-117 kDa and 200-230 kDa, respectively.

Formulation, Preparation and Storage

10263-S4
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 4B

Semaphorin 4B (Sema4B) is a 95-100 kDa, class IV member of the Semaphorin family of proteins (1). Mature mouse Sema4B is a type I transmembrane glycoprotein that is 793 amino acids (aa) in length. It contains a 673 aa extracellular domain (ECD) that is characterized by one Sema domain, a PSI region, and an Ig-like C2-type domain. Within the ECD, mouse Sema4B shares 85% and 95% aa identity with the human and rat Sema4B, respectively (1, 2). Sema4B is expressed in hippocampal neurons, glial cells, and immune cells (3-4). It colocalizes and interacts with PSD-95 and participates in the formation or functioning of glutamatergic synapses (4). Sema4B-/- mice display reduced proliferation of astrocytes after CNS injury (5). In the immune system, Sema4B is a negative regulator of basophil-mediated immune response and is implicated in the development of lung cancer (6, 7).

References

  1. Gurrapu, S. et al. (2016) Cell Adh. Migr. 10:675.
  2. Alto, L. and J.R. Terman (2017) Methods. Mol. Biol. 1493:1.
  3. Schultze, W. et al. (2001) J. Neurochem. 78:482.
  4. Burkhardt, C. et al. (2005) FEBS Lett. 579:3821.
  5. Ben-Gigi, L. et al. (2015) eNeuro. 2:1.
  6. Nakagawa, Y. et al. (2011) J. Immunol. 186:2881.
  7. Nagai, H. et al. (2007) Oncogene. 26:4025.

Alternate Names

SEMA4B, SEMAC, SemC

Entrez Gene IDs

10509 (Human); 20352 (Mouse); 293042 (Rat)

Gene Symbol

SEMA4B

UniProt

Q62179.2

Additional Semaphorin 4B Products

Product Documents for Recombinant Mouse Semaphorin 4B Fc Chimera Protein, CF

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