Recombinant Mouse Semaphorin 6B Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2264-S6

R&D Systems, part of Bio-Techne
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2264-S6-050
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Key Product Details

Source

HEK293

Accession #

O54951

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Semaphorin 6B Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived mouse Semaphorin 6B protein
Mouse Semaphorin 6B
(Leu27-Ser605)
Accession # O54951		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu27

Predicted Molecular Mass

91 kDa (monomer)

SDS-PAGE

100-115 kDa, reducing conditions

Activity

Measured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones.
5 μg/mL of Recombinant Mouse Semaphorin 6B Fc Chimera causes >50% growth cone collapse in the presence of 10 ng/mL of Recombinant Human beta-NGF (Catalog # 256-GF).

Formulation, Preparation and Storage

2264-S6
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Tween® 20.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 6B

Semaphorin 6B is a member of the class 6 subfamily of semaphorins, a large, highly conserved family of signaling molecules that affect multiple processes including axon guidance, cell migration, synaptogenesis, and dendritic spine formation (1). The class 6 semaphorins are type I transmembrane glycoproteins that contain the characteristic extracellular beta propeller-structured N-terminal semaphorin (sema) domain and also an extracellular plexin-semaphorin-integrin (PSI) domain (2, 3). Additionally, the cytoplasmic domain of Semaphorin 6B contains a proline-rich sequence that has been shown to interact with the SH3 domain of the Src signaling protein (4). Mouse Semaphorin 6B has a molecular weight of approximately 120 kDa and is thought to form disulfide-linked homodimers (4). It shares 95% and 99% amino acid (aa) sequence identity with the human and rat orthologs, respectively. In mice, Semaphorin 6B is highly expressed in developing muscle and in subregions of the nervous system, as well as at low levels in most adult tissues (4). It has been shown to mediate repulsion of mouse sympathetic ganglion axons and hippocampal mossy fibers via binding to the transmembrane protein Plexin A4 (5, 6). Semaphorin 6B has also been shown to be highly expressed in human gastric, breast, and glioblastoma cancer cells and is thought to be involved in tumor differentiation and metastasis (7-9).

References

  1. Pasterkamp, R.J. and R.J. Giger (2009) Curr. Opin. Neurobiol. 19:263.
  2. Neufeld, G. et al. (2005) Front. Biosci. 10:751.
  3. Roney, K. et al. (2013) Protein Cell 4:17.
  4. Eckhardt, F. et al. (1997) Mol. Cell. Neurosci. 9:409.
  5. Suto, F. et al. (2005) J. Neurosci. 25:3628.
  6. Tawarayama, H. et al. (2010) J. Neurosci. 30:7049.
  7. Correa, R.G. et al. (2001) Genomics 73:343.
  8. Murad, H. et al. (2006) Int. J. Oncol. 28:977.
  9. Ge, C. et al. (2013) J. Int. Med. Res. 41:284.

Alternate Names

Sema6B

Entrez Gene IDs

10501 (Human); 20359 (Mouse)

Gene Symbol

SEMA6B

UniProt

O54951

Additional Semaphorin 6B Products

Product Documents for Recombinant Mouse Semaphorin 6B Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse Semaphorin 6B Fc Chimera Protein, CF

For research use only

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