Recombinant Mouse Serpin D1/Heparin Cofactor II Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 1265-PI

R&D Systems, part of Bio-Techne
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1265-PI-010
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Key Product Details

Source

NS0

Accession #

P49182

Conjugate

Unconjugated

Applications

Inhibition Activity

View all Serpin D1/Heparin Cofactor II Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Serpin D1/Heparin Cofactor II protein
Glu24-Ser478, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu24

Predicted Molecular Mass

53 kDa

SDS-PAGE

70-80 kDa, reducing conditions

Activity

Measured by its ability to inhibit Recombinant Human Coagulation Factor II/Thrombin (Catalog # 1473-SE) cleavage of a fluorogenic peptide substrate Boc-VPR-AMC (Catalog # ES011).
The IC50 value is <1.2 nM, as measured under the described conditions.

Formulation, Preparation and Storage

1265-PI
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile 25 mM HEPES and 150 mM NaCl, pH 7.4.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin D1/Heparin Cofactor II

Serpin D1 is a member of the Serpin superfamily of the serine protease inhibitors (1). Similar to Serpins A5 and C1, it inhibits thrombin and this activity is enhanced by heparin. Interestingly, a C-terminal His-tagged recombinant Serpin D1 had enhanced heparin effect, which was maintained in a plasma-based thrombin inhibition assay (2). Congenital Serpin D1 deficiency is a potential risk factor for thrombosis (3). Mouse Serpin D1 is synthesized as a 478 amino acid precursor with a 23 amino acid signal peptide (4). The mature chain (residues 24 to 478) is secreted.

References

  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Bauman, S.J. et al. (1999) J. Biol. Chem. 274:34556.
  3. Tollefsen, D.M. (2002) Arch. Pathol. Lab. Med. 126:1394.
  4. Zhang, G.S. et al. (1994) Biochemistry 33:3632.

Alternate Names

HC2, HCF2, Heparin Cofactor II, LS2

Entrez Gene IDs

3053 (Human); 15160 (Mouse)

Gene Symbol

SERPIND1

UniProt

P49182

Additional Serpin D1/Heparin Cofactor II Products

Product Documents for Recombinant Mouse Serpin D1/Heparin Cofactor II Protein, CF

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Product Specific Notices for Recombinant Mouse Serpin D1/Heparin Cofactor II Protein, CF

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