Recombinant Mouse SEZ6L Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4804-S6

R&D Systems, part of Bio-Techne
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4804-S6-050
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Key Product Details

Source

NS0

Accession #

NP_064366

Conjugate

Unconjugated

Applications

Bioactivity

View all SEZ6L Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse SEZ6L protein
Glu32-Ser889, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu32

Predicted Molecular Mass

94 kDa

SDS-PAGE

115-185 kDa, reducing conditions

Activity

Measured by its ability to induce cell clumping and inhibit neurite outgrowth of Neuro-2A mouse neuroblastoma cells.
2 μg/mL of Recombinant Mouse SEZ6L is able to signifcantly induce cell clumping and inhibit neurite outgrowth.

Formulation, Preparation and Storage

4804-S6
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SEZ6L

Seizure 6-like Protein (SEZ6L), also known as Brain-specific Receptor-like Protein B (BSRP-B), is a member of the SEZ6 family of proteins with a molecular weight of approximately 200 kDa (1). Mouse SEZ6L is a type I transmembrane glycoprotein composed of an extracellular domain (ECD) that contains three CUB domains and five short consensus repeats (SCRs) (1). Alternative splicing generates an isoform that contains a phenylalanine substitution for amino acids (aa) 807-872 of the fifth SCR domain (1). Mouse SEZ6L shares 80% and 97% aa sequence identity with the human and rat orthologs, respectively. It is expressed in the plasma membrane and endoplasmic reticulum of neurons and lung bronchiolar epithelium and likely regulates PKC alpha activation (1, 2). In mouse pancreatic beta cells, SEZ6L has been shown to be a substrate for BACE-2, which cleaves its ECD at a site 16 aa upstream from its transmembrane domain (3). Additionally, human SEZ6L has been shown to be involved in the maintenance of cellular cholesterol homeostasis (4). BSRP-C also known as SEZ6, has been reported to inhibit neurite outgrowth in mice (5). R&D Systems in-house testing shows that mouse SEZ6L, also exhibits this function. Genetic variations in human Sez6l have been associated with bipolar disorder I and an increased risk for lung cancer (2, 6, 7).

References

  1. Miyazaki, T. et al. (2006) FEBS Lett. 580:4057.
  2. Nishioka, M. et al. (2000) Oncogene 19:6251.
  3. Stützer, I. et al. (2013) J. Biol. Chem. 288:10536.
  4. Blattmann, P. et al. (2013) PLoS Genetics 9:e1003338.
  5. Gunnersen, J.M. et al. (2007) Neuron 56:621.
  6. Gorlov, I.P. et al. (2007) Cancer Res. 67:8406.
  7. Xu, C. et al. (2013) J. Affect. Disord. 145:95.

Long Name

Seizure-related 6 Homolog-like

Alternate Names

Acig1, AIG1, Aig1L, BSRP-B, SEZ6L

Entrez Gene IDs

23544 (Human); 56747 (Mouse); 304554 (Rat)

Gene Symbol

SEZ6L

UniProt

NP_064366

Additional SEZ6L Products

Product Documents for Recombinant Mouse SEZ6L Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse SEZ6L Protein, CF

For research use only

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