Recombinant Mouse SLITRK4 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 1811-SK

R&D Systems, part of Bio-Techne
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1811-SK-050
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Key Product Details

Source

NS0

Accession #

Q810B8

Conjugate

Unconjugated

Applications

Bioactivity

View all SLITRK4 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse SLITRK4 protein
Asp19-Ser618, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp19

Predicted Molecular Mass

68 kDa

SDS-PAGE

90-100 kDa, reducing conditions

Activity

Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons.
Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 200 ng on a nitrocellulose-coated microplate

Scientific Data Images for Recombinant Mouse SLITRK4 His-tag Protein, CF

Recombinant Mouse SLITRK4 His-tag Protein SDS-PAGE

Recombinant Mouse SLITRK4 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse SLITRK4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 90-100 kDa.

Formulation, Preparation and Storage

1811-SK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: SLITRK4

SLITRK4 is an approximately 90 kDa type I transmembrane member of the SLITRK family of proteins which contain a slit-like extracellular region and a Trk-like cytoplasmic region (1). Within the extracellular domain (ECD), the mouse SLITRK4 shares 98% amino acid sequence identity with human SLITRK4. The ECD of SLITRK4 contains 6 leucine rich repeats (LRR) followed by a C-terminal LRR domain, and 6 more LRRs flanked by a pair of N- and C-terminal LRR domains (2, 3). SLITRK4 is expressed in the testis and multiple regions of the brain, particularly the cerebral cortex, cerebellum, and hippocampus (4). SLITRK4 can suppress neurite outgrowth and promote the formation of excitatory presynaptic structures (3, 5). SLITRK genes are also differentially expressed in brain tumors, including astrocytoma, oligodendroglioma, glioblastoma, medulloblastoma, and supratentorial primitive neuroectodermal tumor (PNET) (2).

References

  1. Ko, J. (2012) Mol. Cells 34:335.
  2. Aruga, J. et al. (2003) Gene 315:87.
  3. Aruga, J. and K. Mikoshiba (2003) Mol. Cell Neurosci. 24:117.
  4. Yim, Y.S. et al. (2013) Proc. Natl. Acad. Sci. USA 110:4057.
  5. Takahashi, H. et al. (2012) Nat. Neurosci. 15:389.

Long Name

SLIT and NTRK-like Family, Member 4

Alternate Names

DKFZp547M2010, SLIT and NTRK-like family, member 4, SLIT and NTRK-like protein 4, slit and trk like gene 4

Entrez Gene IDs

139065 (Human); 245446 (Mouse)

Gene Symbol

SLITRK4

UniProt

Q810B8

Additional SLITRK4 Products

Product Documents for Recombinant Mouse SLITRK4 His-tag Protein, CF

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Product Specific Notices for Recombinant Mouse SLITRK4 His-tag Protein, CF

For research use only

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