Recombinant Mouse Sphingosine Kinase 1/SPHK1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6086-SK

R&D Systems, part of Bio-Techne
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6086-SK-010
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Q8CI15

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Sphingosine Kinase 1/SPHK1 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Sphingosine Kinase 1/SPHK1 protein
Glu2-Pro382, with an N-terminal Met and 6-His tag

Purity

>70%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Inconclusive result, Met predicted. Protein identity confirmed by MS analysis of tryptic fragments.

Predicted Molecular Mass

43 kDa

SDS-PAGE

42 kDa, reducing conditions

Activity

Measured by its ability to phosphorylate Omega-(7-nitro-2-1,3-benzoxadiazol-4-yl)-D-erythro-sphingosine (NBD-sphingosine).
The specific activity is >2,500 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6086-SK
Formulation Supplied as a 0.2 μm filtered solution in MES, NaCl, Glycerol and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Sphingosine Kinase 1/SPHK1

Sphingosine kinases are cytosolic or membrane-associated enzymes that catalyze the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P). Two types of sphingosine kinases, SPHK1 and SPHK2, are known to be expressed in human cells. The two enzymes share considerable amino acid sequence similarity, but differ in their N‑terminal and central regions (1). The two proteins also differ in tissue distribution and some kinetic properties (1). S1P is a lipid messenger that regulates diverse physiological processes including cell proliferation, migration, apoptosis, inflammation, calcium homeostasis and cytoskeletal structure (2, 3). The level of S1P is tightly controlled by SPHKs and S1P degrading enzymes. SPHK1 and its activation can be stimulated by several growth factors such as tumor necrosis factor-alpha, epidermal growth factor and transforming growth factor-beta (3, 4). Expression of SPHK1 has been found to increase in many human solid tumors and overexpression of SPHK1 is associated with tumor angiogenesis (5). Such studies have implicated SPHK1 as a new target for cancer treatment.

References

  1. Liu, H. et al. (2000) J. Biol. Chem. 275:19513.
  2. Spiegel, S. (1999) J. Leukocyte Biol. 65:341.
  3. Alemany, R. et al. (2007) Naunyn-Schmiedegerg’s Arch. Pharmacol. 374:413.
  4. Pederson, L. et al. (2008) Proc. Natl. Acad. Scis USA. 105:20764.
  5. Shida, D. et al. (2008) Curr. Drug Targets. 9:662.

Alternate Names

SK1, SPK1

Entrez Gene IDs

8877 (Human); 20698 (Mouse); 170897 (Rat)

Gene Symbol

SPHK1

UniProt

Q8CI15

Additional Sphingosine Kinase 1/SPHK1 Products

Product Documents for Recombinant Mouse Sphingosine Kinase 1/SPHK1 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Sphingosine Kinase 1/SPHK1 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd. Triton is a registered trademark of Union Carbide Corp.

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