Recombinant Mouse SR-BI Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9644-SR

R&D Systems, part of Bio-Techne
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9644-SR-050
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Key Product Details

Source

HEK293

Accession #

Q61009-1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all SR-BI Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived mouse SR-BI protein
Mouse SR-BI
(Pro33-Tyr443)
Accession # Q61009-1		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro33

Predicted Molecular Mass

74 kDa

SDS-PAGE

85-120 kDa, reducing conditions

Activity

Measured by its ability to bind fluorescein-conjugated S. aureus Bioparticles. Jiang, Y. et al. (2006) J. Biol. Chem. 281:11834.
The ED50 for this effect is 0.4-2 μg/mL.

Formulation, Preparation and Storage

9644-SR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SR-BI

Scavenger Receptor, class B, member 1 (SR-BI), gene name SCARB1, is also known as CD36L1 (CD36-like 1) or CLA-1 (CD36 and LIMPII analogous 1) (1-5). SR-BI is a transmembrane glycoprotein found on macrophages, liver cells and other steroidogenic cells as a lipoprotein receptor. The 509 amino acid (aa) mouse SR-BI contains a central extracellular domain (ECD), flanked by N- and C-terminal transmembrane domains. A mouse splice variant differs at the C-terminal cytoplasmic domain (SR-BII, 506 aa) (2). The mouse SR-BI ECD shares 81% and 92% aa sequence identity with human and rat SR-BI, respectively. SR-BI functions in reverse cholesterol transport (RCT), which is thought to be anti-atherogenic by facilitating transport of cholesteryl esters from macrophages back to the liver for degradation (3). In rodent hepatocytes, SR-BI is the main receptor mediating RCT, while human hepatocytes also express a second mediator, CETP (cholesteryl ester transfer protein) (3-5). On endothelial cells, signaling through SR-BI activates nitric oxide production, which attenuates monocyte adhesion (6). On adrenocortical cells, SR-BI mediates uptake of cholesteryl esters from HDL for the synthesis of glucocorticoid hormones such as cortisol (3-5). On platelets, HDL binding to surface SR-BI inhibits aggregation and increases platelet survival time (3-5). SR-BI and its SR-BII isoform also bind bacterial lipopolysaccharides, facilitating uptake of various bacteria by cells such as peritoneal macrophages (7, 8). This uptake enhances inflammatory responses which, unless properly controlled, can result in sepsis (8-10).

References

  1. Calvo, D. and M. A. Vega (1993) J. Biol. Chem. 268:18929.
  2. Webb, N.R. et al. (1998) J. Biol. Chem. 273:15241.
  3. Chadwick, A.C. and D. Sahoo (2013) Curr. Opin. Endocrinol. Diabetes Obes. 20:124.
  4. Hoekstra, M. et al. (2012) Curr. Opin. Lipidol. 23:127.
  5. Vergeer, M. et al. (2011) N. Engl. J. Med. 364:136.
  6. Guo, L. et al. (2011) J. Lipid Res. 52:2272.
  7. Vishnyakova, T.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:16888.
  8. Baranova, I.N. et al. (2012) J. Immunol. 188:1371.
  9. Leelahavanichkul, A. et al. (2012) J. Immunol. 188:2749.
  10. Guo, L. et al. (2009) J. Biol. Chem. 284:19826.    

Long Name

Scavenger Receptor Class B, Member I

Alternate Names

CD36L1, CLA1, HDLQTL6, SCARB1, SR-B1, SRBI

Entrez Gene IDs

949 (Human); 20778 (Mouse); 25073 (Rat)

Gene Symbol

SCARB1

UniProt

Q61009-1

Additional SR-BI Products

Product Documents for Recombinant Mouse SR-BI Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse SR-BI Fc Chimera Protein, CF

For research use only

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