Recombinant Mouse ST2/IL-33R His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10695-MR

R&D Systems, part of Bio-Techne
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10695-MR-050
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Key Product Details

Source

CHO

Accession #

P14719.2

Conjugate

Unconjugated

Applications

Bioactivity

View all ST2/IL-33R Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse ST2/IL-33R protein
Ser27-His331, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser27

Predicted Molecular Mass

36 kDa

SDS-PAGE

57-68 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse ST2/IL-33R His-tag (Catalog # 10695-MR) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Mouse IL-33  Recombinant Mouse IL‑33 (Catalog # 3626-ML) binds with an ED50 of 0.03-0.27 µg/mL.

Scientific Data Images for Recombinant Mouse ST2/IL-33R His-tag Protein, CF

Recombinant Mouse ST2/IL-33R His-tag Protein Binding Activity.

When Recombinant Mouse ST2/IL-33R His-tag (Catalog # 10695-MR) is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Mouse IL-33 (3626-ML) binds with an ED50 of 0.03-0.27 μg/mL.

Recombinant Mouse ST2/IL-33R His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Mouse ST2/IL-33R His-tag (Catalog # 10695-MR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 57-68 kDa.

Formulation, Preparation and Storage

10695-MR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ST2/IL-33R

ST2, also known as IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Mouse ST2 consists of a 306 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 23 aa transmembrane segment, and a 212 aa cytoplasmic domain with an intracellular TIR domain (3). Alternate splicing of the 120 kDa mouse ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions (3). Within the ECD, mouse ST2 shares 59% and 81% aa sequence identity with human and rat ST2, respectively. ST2 is believed to be a useful biomarker for predicting both acute and chronic heart failure, and is expressed mainly in two isoforms, as a transmembrane (ST2L) and as a soluble form (sST2) (4,5). ST2L is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (6-9). It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (6, 10). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 11, 12). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (8). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (6, 13-15). ST2 is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (7, 9, 16). Level of ST2 expression is associated with gastric cancer progression (17). When bound to IL-33, ST2 is indicated as an immunotherapy target for diagnosis and treament of cancer patients likely to be resistant to conventional treatments (18). Soluble ST2 functions as a decoy receptor that blocks IL-33's ability to signal through transmembrane ST2 (11, 14-16).

References

  1. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
  2. Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
  3. Yanagisawa, K. et al. (1993) FEBS Lett. 318:83.
  4. Pascual-Figal DA et al. (2015) Am J Cardiol 115(7 Suppl):3B-7B.
  5. Villacorta, Humberto et al. (2016) Arquivos brasileiros de cardiologia vol. 106,2.
  6. Schmitz, J. et al. (2005) Immunity 23:479.
  7. Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
  8. Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
  9. Weinberg, E.O. et al. (2002) Circulation 106:2961.
  10. Sanada S. et al. (2007) J. Clin. Invest. 117:1538.
  11. Palmer, G. et al. (2008) Cytokine 42:358.
  12. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
  13. Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
  14. Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
  15. Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
  16. Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.
  17. Bai, F. et al. (2019) Oncol Lett. 17:5761.
  18. Kudo-Saito, C. et al. (2020) Cancer Res. 80:1981.

Long Name

Interleukin 33 Receptor

Alternate Names

DER4, Fit-1, IL-1 R4, IL-1R4, IL-1RL1, IL-33 R, IL-33R, IL1R4, IL33R, Ly84, ST2L, ST2V, T1

Entrez Gene IDs

9173 (Human); 17082 (Mouse)

Gene Symbol

IL1RL1

UniProt

P14719.2

Additional ST2/IL-33R Products

Product Documents for Recombinant Mouse ST2/IL-33R His-tag Protein, CF

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Product Specific Notices for Recombinant Mouse ST2/IL-33R His-tag Protein, CF

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