Recombinant Mouse TCAM-1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3546-TC
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse TCAM-1 protein
Ala20-Gly483 & Glu22-Gly483
Ala20-Gly483 & Glu22-Gly483
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala20 & Glu22
Predicted Molecular Mass
52 kDa
SDS-PAGE
70-80 kDa, reducing conditions
Activity
Measured by the ability of the immobilized protein to support the adhesion of MLTC-1 mouse Leydig tumor cells.
When 5 x 104 cells per well are added to TCAM-1 coated plates (5 μg/mL, 100 μL/well), approximately 65%-85% will adhere after 45 minutes at 37 °C.
When 5 x 104 cells per well are added to TCAM-1 coated plates (5 μg/mL, 100 μL/well), approximately 65%-85% will adhere after 45 minutes at 37 °C.
Formulation, Preparation and Storage
3546-TC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 200 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: TCAM-1
Mouse TCAM-1 (Testicular cell adhesion molecule-1) is a 59 kDa (predicted) type I transmembrane protein that is a member of the intracellular adhesion molecule (ICAM) family belonging to the immunoglobulin (Ig) superfamily (1). It is synthesized from a 548 amino acid (aa) precursor that contains an 18 aa signal sequence, a 465 aa extracellular region, a 25 aa transmembrane region, and a short cytoplasmic tail of 40 aa. The extracellular region contains five Ig domains and six potential N-linked glycosylation sites (1). Mouse TCAM-1 is 89% identical to rat TCAM-1. Expression of TCAM-1 is detected in the embryo on day 17 and in adult testis, and correlates with the presence of pachytene spermatocytes and spermatids in mouse testis development (1).
References
- Skatani, S. et al. (2000) Genome 43:957.
Long Name
Testicular Cell Adhesion Molecule 1, TCAM-1P
Alternate Names
TCAM1
Gene Symbol
TCAM1P
UniProt
Additional TCAM-1 Products
Product Documents for Recombinant Mouse TCAM-1 Protein, CF
Product Specific Notices for Recombinant Mouse TCAM-1 Protein, CF
For research use only
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