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Recombinant Mouse TLR9 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7960-TR

R&D Systems, part of Bio-Techne
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7960-TR-050

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse TLR9 protein
Mouse TLR9
(Leu26-Asp818)
Accession # AAK29625
IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu26

Predicted Molecular Mass

115.8 kDa (monomer)

SDS-PAGE

140-170 kDa, reducing conditions

Activity

Measured by its ability to bind biotinylated CpG oligodeoxynucleotide, type B. The 50% maximum binding is achieved at 30‑150 ng/mL of Recombinant Mouse TLR9 Fc Chimera.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

7960-TR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR9

TLR9 (Toll‑like receptor 9), designated CD289, is a member of the TLR family of innate immune receptors that is mainly expressed by colonic epithelium, CD123+ plasmacytoid predendritic cells (pDC), and splenic transitional B cells (1‑9). TLR9 responds to unmethylated DNA CpG motifs that occur mainly in bacteria and viruses (1, 2). Mouse TLR9 cDNA encodes a 1032 amino acid (aa) type I transmembrane glycoprotein with a 793 aa extracellular domain (ECD) that contains 26 leucine‑rich repeats (LRRs, aa 26‑818), and a 193 aa cytoplasmic domain with a TIR sequence that dimerizes with signaling adaptors such as MyD88 (1). The mouse TLR9 ECD shares 87% aa sequence identity with rat and 71‑74% with human, feline, canine, equine, porcine, bovine and ovine TLR9. Predicted splice forms vary at the N‑terminus by initiating either upstream or downstream of the standard site. The full-length 150 kDa form, which is ligand‑binding but nonsignaling, is found in the endoplasmic reticulum. It undergoes accessory protein-mediated translocation either to the cell membrane or to lysosomes (1‑3). TLR9 is cleaved to remove LRR1‑14, producing an 80 kDa signaling fragment within acidic endolysosomes where it encounters microbial CpG DNA rather than self-DNA (2, 10, 11). However, immune complexes of self‑DNA with lupus erythematosus anti‑DNA antibodies can induce TLR9 activation and IFN‑ alpha production in pDC (4). A soluble form also found in endosomes includes all 26 LRRs and negatively regulates active TLR9 (12). Activation of TLR9 contributes to splenocyte proliferation, pDC maturation, macrophage inflammatory cytokine production, Th1 inflammatory responses, NK cell activation and recruitment, B cell surface MHC class II up‑regulation and immunoglobulin production, and generation and maintenance of memory B cells (1, 5‑9).

References

  1. Hemmi, H. et al. (2000) Nature 408:740.
  2. Barton, G.M. et al. (2006) Nat. Immunol. 7:49.
  3. Latz, E. et al. (2004) Nat. Immunol. 5:190.
  4. Barrat, F.J. et al. (2005) J. Exp. Med. 202:1131.
  5. Krieg, A.M. et al. (1995) Nature 374:546.
  6. Aranburu, A. et al. (2010) J. Immunol. 185:7293.
  7. Guerrier, T. et al. (2012) J. Autoimmun. 39:173.
  8. Guillerey, C. et al. (2012) Blood 120:90.
  9. Cunningham-Rundles, C. et al. (2006) J. Immunol. 176:1978.
  10. Ewald, S.E. et al. (2008) Nature 456:658.
  11. Park, B. et al. (2008) Nat. Immunol. 9:1407.
  12. Chockalingam, A. et al. (2011) Eur. J. Immunol. 41:2176.

Long Name

Toll-like Receptor 9

Alternate Names

CD289

Entrez Gene IDs

54106 (Human); 81897 (Mouse); 338457 (Rat)

Gene Symbol

TLR9

UniProt

Additional TLR9 Products

Product Documents for Recombinant Mouse TLR9 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse TLR9 Fc Chimera Protein, CF

For research use only

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