Recombinant Mouse Tryptase-5/Prss32 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2634-SE
Key Product Details
Source
NS0
Accession #
Structure / Form
Pro form
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Tryptase-5/Prss32 protein
Ser20-Arg304, with a C-terminal 10-His tag
Ser20-Arg304, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser20
Predicted Molecular Mass
32 kDa
SDS-PAGE
40 kDa doublet, reducing conditions
Activity
Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Arg-ThioBenzyl ester (Z-R-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.
The specific activity is >400 pmol/min/µg, as measured under the described conditions.
The specific activity is >400 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
2634-SE
| Formulation | Lyophilized from a 0.2 μm filtered solution in MES and NaCl. |
| Reconstitution | Reconstitute at 200 μg/mL in sterile 50 mM Tris, 10 mM CaCl2, 150 mM NaCl and 0.05% Brij-35 pH 7.5. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Tryptase-5/Prss32
Tryptase-5 is encoded by Prss32, one of 13 genes on mouse chromosome 17A3.3 that correspond to functional trypsin-like serine proteases (1). The deduced amino acid sequence of mouse Tryptase-5 consists of 331 residues with a signal peptide (residues 1 to 19), a pro region (residue 20 to 53), a catalytic domain (54 to 304), and a C-terminal hydrophobic peptide (residues 305 to 331). The mRNA is expressed in smooth muscle, eye, stomach, uterus and lymph node. Apparently, Prss32 does not seem to have a counterpart in the human genome. The mouse Tryptase-5 (residues 20 to 304) was expressed in the NS0 cells with a foreign signal peptide. The protein was secreted and purified. After being treated by thermolysin, the enzyme is active against a peptide substrate as described in the Activity Assay Protocol.
References
- Wong, G. W. et al. (2004) J. Biol. Chem. 279:2438.
Alternate Names
Prss32, Tryptase5
Entrez Gene IDs
69814 (Mouse)
Gene Symbol
PRSS32
UniProt
Additional Tryptase-5/Prss32 Products
Product Documents for Recombinant Mouse Tryptase-5/Prss32 Protein, CF
Product Specific Notices for Recombinant Mouse Tryptase-5/Prss32 Protein, CF
For research use only
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