Recombinant Mouse Tyro3/Dtk Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 759-DT
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Binding Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Tyro3/Dtk protein
| Mouse Tyro3/Dtk (Ala31-Ser418) Accession # P55144 |
DIEGRMD | Human IgG1 (Pro100-Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala31
Predicted Molecular Mass
69 kDa (monomer)
SDS-PAGE
100-110 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Mouse Tyro3/Dtk Fc Chimera at 5 µg/mL (100 µL/well) can bind Recombinant Human Gas6 (Catalog # 885-GSB) with a linear range of 3‑200 ng/mL.
Immobilized Recombinant Mouse Tyro3/Dtk Fc Chimera at 5 µg/mL (100 µL/well) can bind Recombinant Human Gas6 (Catalog # 885-GSB) with a linear range of 3‑200 ng/mL.
Formulation, Preparation and Storage
759-DT
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Tyro3/Dtk
Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.
References
- Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
- Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Long Name
Tyrosine Protein Kinase Receptor TYRO3
Alternate Names
Brt, Dtk, Rse, Sky
Gene Symbol
TYRO3
UniProt
Additional Tyro3/Dtk Products
Product Documents for Recombinant Mouse Tyro3/Dtk Fc Chimera Protein, CF
Product Specific Notices for Recombinant Mouse Tyro3/Dtk Fc Chimera Protein, CF
For research use only
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