Recombinant Mouse Tyro3/Dtk His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10480-DT

R&D Systems, part of Bio-Techne
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10480-DT-100
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Key Product Details

Source

NS0

Accession #

P55144.2

Conjugate

Unconjugated

Applications

Bioactivity

View all Tyro3/Dtk Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Tyro3/Dtk protein
Ala31-Thr418, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala31

Predicted Molecular Mass

42 kDa

SDS-PAGE

60-75 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Tyro3/Dtk His-tag (Catalog # 10480-DT) is immobilized at 1 µg/mL (100 µL/well), Recombinant Mouse Gas6 (Catalog # 8310-GS) binds with an ED50 of 0.4-2.4 μg/mL.

Scientific Data Images for Recombinant Mouse Tyro3/Dtk His-tag Protein, CF

Recombinant Mouse Tyro3/Dtk His-tag Protein Binding Activity

Recombinant Mouse Tyro3/Dtk His-tag Protein Binding Activity

When Recombinant Mouse Tyro3/Dtk His-tag Protein is immobilized at 1 μg/mL (100 μL/well), Recombinant Mouse GAS6 (Full Length) Protein (Catalog # 8310-GS) binds with an ED50 of 0.4-2.4 μg/mL.
Recombinant Mouse Tyro3/Dtk His-tag Protein SDS-PAGE

Recombinant Mouse Tyro3/Dtk His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse Tyro3/Dtk His-tag Protein (Catalog # 10480-DT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-75 kDa.

Formulation, Preparation and Storage

10480-DT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tyro3/Dtk

Dtk (Sky, Tyro3, Rse, Brt), Axl (Ufo, Ark), and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. Mouse DTK is a 42kDa protein with a 30 amino acid (aa) signal sequence, 389 aa extracellular domain (ECD), and a 20 aa transmembrane segment. The ECD of mouse DTK shares 85% and 96% sequence identity with the ECD of human and rat respectively. The extracellular domain of these proteins contains two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain. In embryoid stem cells growing in the presence of leukemia inhibitory factor, DTK is abundantly expressed and this level of expression is maintained in differentiating embryoid stem cells and cystic embryoid bodies. In mid-gestational embryos, DTK RNA is expressed in many tissues including brain, eye, thymus, lung, heart, gut, liver, testis and limbs. In contrast, expression of DTK in adult mice becomes restricted to brain, portions of gastrointestinal tract, bladder, testis and ovary.  Overexpression of DTK and several other receptor tyrosine kinase have been found in small cell lung cancer (4).

References

  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
  3. Crosier, P. et al. (1994) Growth Factors 11:125.
  4. Hamilton, G. et al. (2015) Oncoscience 2:629.

Long Name

Tyrosine Protein Kinase Receptor TYRO3

Alternate Names

Brt, Dtk, Rse, Sky

Entrez Gene IDs

7301 (Human); 22174 (Mouse); 102135978 (Cynomolgus Monkey)

Gene Symbol

TYRO3

UniProt

P55144.2

Additional Tyro3/Dtk Products

Product Documents for Recombinant Mouse Tyro3/Dtk His-tag Protein, CF

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Product Specific Notices for Recombinant Mouse Tyro3/Dtk His-tag Protein, CF

For research use only

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