Recombinant Mouse VEGFR2/Flk-1 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10095-KD

R&D Systems, part of Bio-Techne
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10095-KD-050
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Key Product Details

Source

NS0

Accession #

P35918

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Bioactivity

View all VEGFR2/KDR/Flk-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse VEGFR2/KDR/Flk-1 protein
Ala20-Glu762, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala20

Predicted Molecular Mass

844 kDa

SDS-PAGE

120-132 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse VEGFR2/KDR/Flk-1 His-tag (Catalog # 10095-KD) is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Recombinant Mouse VEGF 164 (Catalog # 493-MV) that produces 50% of the optimal binding response is 0.015-0.12 μg/mL.

Scientific Data Images for Recombinant Mouse VEGFR2/Flk-1 His-tag Protein, CF

Recombinant Mouse VEGFR2/Flk-1 His-tag Protein Binding Activity

Recombinant Mouse VEGFR2/Flk-1 His-tag Protein Binding Activity

When Recombinant Mouse VEGFR2/KDR/Flk-1 (Catalog # 10095-KD) is immobilized at 0.5 μg/mL, 100 μL/well, Recombinant Mouse VEGF 164 (Catalog # 493-MV) binds with an ED50 of 0.015-0.12 μg/mL.
Recombinant Mouse VEGFR2/Flk-1 His-tag Protein SDS-PAGE

Recombinant Mouse VEGFR2/Flk-1 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse VEGFR2/KDR/Flk‑1 His-tag (Catalog # 10095-KD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 120-132 kDa.

Formulation, Preparation and Storage

10095-KD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR2/KDR/Flk-1

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and a tyrosine kinase domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature mouse VEGFR2 is composed of a 743aa residue extracellular domain, a 21 aa residue transmembrane domain and a 584 aa residue cytoplasmic domain. The mature mouse VEGFR2 shares 80% and 93% sequence identity to human and rat VEGFR2. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2 binds VEGF with high affinity and is a potent VEGF antagonist (1, 2).

References

  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
  2. Achen, M.G. et al. (1998) Proc. Natl. Acad. Sci. USA. 95:548.

Long Name

Vascular Endothelial Growth Factor Receptor 2

Alternate Names

CD309, Flk-1, Flk1, KDR, KRD1, Ly73, VEGF R2

Entrez Gene IDs

3791 (Human); 16542 (Mouse)

Gene Symbol

KDR

UniProt

P35918

Additional VEGFR2/KDR/Flk-1 Products

Product Documents for Recombinant Mouse VEGFR2/Flk-1 His-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse VEGFR2/Flk-1 His-tag Protein, CF

For research use only

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