Recombinant Mouse VSIG2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9598-VS

R&D Systems, part of Bio-Techne
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9598-VS-050
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Key Product Details

Source

NS0

Accession #

Q9Z109-1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all VSIG2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse VSIG2 protein
Mouse VSIG2
(Val25-Ala244)
Accession # Q9Z109-1		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val25

Predicted Molecular Mass

50 kDa

SDS-PAGE

58-66 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-17 secretion by human peripheral blood mononuclear cells (PBMC).
The ED50 for this effect is 0.75-4.5 μg/mL.

Scientific Data Images for Recombinant Mouse VSIG2 Fc Chimera Protein, CF

Recombinant Mouse VSIG2 Fc Chimera Protein Bioactivity

Recombinant Mouse VSIG2 Fc Chimera Protein Bioactivity

Recombinant Mouse VSIG2 Fc Chimera (Catalog # 9598‑VS) inhibits IL-17 secretion by human peripheral blood mononuclear cells (PBMC), in the presence of anti-CD3 antibody. The ED50 for this effect is 0.75-4.5 μg/mL.

Formulation, Preparation and Storage

9598-VS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG2

V-set and immunoglobulin domain-containing protein 2 (VSIG2), also known as CTM or CT-like protein, is a member of the much larger Ig superfamily. Mature mouse VSIG2 is a single-pass type I transmembrane protein with a 220 aa extracellular domain (ECD) and a 63 aa cytoplasmic region linked by a short transmembrane domain. The ECD of VSIG2 contains one Ig-like C2-type (immunoglobulin-like) and one Ig-like V-type (immunoglobulin-like) domain. Mouse VSIG2 ECD shares 85% and 94% identity with human and rat, respectively. In humans, VSIG2 is highly expressed in stomach, colon, prostate, trachea and thyroid glands and weakly in bladder and lung (1). V-set domains are Ig-like domains resembling the antibody variable domain and are found in diverse protein families from immunoglobulin light and heavy chains to T cell receptors and adhesion molecules (2). Several VSIG molecules, including VSIG1 and VSIG4, can be classified into the junctional adhesion molecule (JAM) family based on the immunoglobulin folds, V‐ and C2‐types, found in the ECD (3, 4). While the function of VSIG2 is currently unknown, based on the structural similarity to JAM molecules, it is most likely a cell adhesion molecule (2-4). R&D Systems in-house testing indicates that VSIG2 inhibits anti-CD3 antibody induced IL-17 secretion in human PBMC.

Long Name

V-Set and Immunoglobulin Domain Containing 2

Alternate Names

CTH, CTXL

Entrez Gene IDs

23584 (Human); 57276 (Mouse); 300520 (Rat)

Gene Symbol

VSIG2

UniProt

Q9Z109-1

Additional VSIG2 Products

Product Documents for Recombinant Mouse VSIG2 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Manufacturing Specifications

  1. Chrétien, I. et al. (1998) Eur J Immunol. 28(12):4094.
  2. Barclay, A.N. (2003) Semin Immunol. 15(4):215.
  3. Bazzoni, G. Curr Opin (2003) Cell Biol. 15(5):525.
  4. Zang, X. and Allison, J.P. (2006) J Clin Invest. 116(10):2590.

Product Specific Notices for Recombinant Mouse VSIG2 Fc Chimera Protein, CF

For research use only

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