Recombinant Mouse VSIG8 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9204-VS

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

Q6P3A4

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all VSIG8 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse VSIG8 protein
Mouse VSIG8
(Val22-Gly262)
Accession # Q6P3A4		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val22

Predicted Molecular Mass

54 kDa

SDS-PAGE

56-66 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes.
The ED50 for this effect is 0.5-3 μg/mL.

Scientific Data Images for Recombinant Mouse VSIG8 Fc Chimera Protein, CF

Recombinant Mouse VSIG8 Fc Chimera Protein Bioactivity

Recombinant Mouse VSIG8 Fc Chimera Protein Bioactivity

Recombinant Mouse VSIG-8 Fc Chimera (Catalog # 9204-VS) inhibits anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.5-3 μg/mL.

Formulation, Preparation and Storage

9204-VS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG8

VSIG8 (V-set and immunoglobulin domain containing 8), also known as C1orf204, is an approximately 45 kDa type I transmembrane protein of the B7 family within the Ig superfamily. Mature mouse VSIG8 consists of a 241 amino acid (aa) extracellular domain (ECD) containing two V-type Ig-like domains, a 21 aa transmembrane domain, and a 134 aa cytoplasmic domain. Within the ECD, mouse VSIG8 shares 88% and 95% aa identity with human and rat VSIG8, respectively. Alternative splicing generates an isoform of mouse VSIG8 that lacks most of the first Ig-like domain. VSIG8 was identified from proteomic analysis of human hair shafts (1, 2). It is expressed in the hair follicle and shaft, superficial layers of the nail matrix, and superficial layers of oral epithelium (3). R&D Systems in-house testing indicates that VSIG8 inhibits the production of IL-2 by activated T cells.

References

  1. Rice, R.H. et al. (2010) J. Proteome Res. 9:6752.
  2. Lee, Y.J. et al. (2006) Mol. Cell. Proteomics 5:789.
  3. Rice, R.H. et al. (2011) J. Invest. Dermatol. 131:1936.

Long Name

V-Set and Immunoglobulin Domain Containing 8

Alternate Names

V-set and immunoglobulin domain containing 8

Entrez Gene IDs

391123 (Human); 240916 (Mouse); 289236 (Rat); 102123183 (Cynomolgus Monkey)

Gene Symbol

VSIG8

UniProt

Q6P3A4

Additional VSIG8 Products

Product Documents for Recombinant Mouse VSIG8 Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse VSIG8 Fc Chimera Protein, CF

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