Recombinant Mouse Wnt-16b Protein

R&D Systems, part of Bio-Techne | Catalog # 9148-WN

R&D Systems, part of Bio-Techne
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Carrier Free
9148-WN-025/CF
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With Carrier
9148-WN-025
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Key Product Details

Source

CHO

Accession #

Q9QYS1

Conjugate

Unconjugated

Applications

Bioactivity

View all Wnt-16b Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-16b protein
Asn30-Lys364

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn30

Predicted Molecular Mass

38 kDa

SDS-PAGE

43-63 kDa, reducing conditions

Activity

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells.
The ED50 for this effect is 2-12 μg/mL.

Scientific Data Images for Recombinant Mouse Wnt-16b Protein

Recombinant Mouse Wnt-16b Protein Bioactivity

Recombinant Mouse Wnt-16b Protein Bioactivity

Recombinant Mouse Wnt-16b (Catalog # 9148-WN) induces differentiation of the C3H10T1/2 mouse mesenchymal stem cell line to alkaline phosphatase secreting osteoblast cells. The ED50 for this effect is 2-12 µg/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 9148-WN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 500 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 9148-WN/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-16b

Wnt-16 is a 40 kDa protein within the Wnt family of secreted, highly conserved, cysteine-rich, palmitoylated cell signaling glycoproteins that play important roles in vertebrate developmental pattern formation, cell fate decision, axon guidance, and tumor formation (1-3). Mature mouse Wnt‑16 shares 92% and 97% aa sequence identity with human and rat Wnt‑16, respectively. Wnt-16 is expressed by uterine stromal cells adjacent to the luminal epithelium during implantation (4). It is
up-regulated during the first embryonic lymphoid progenitor differentiation (5). Congenital heart defects correlate with elevated Wnt-16 in mouse embryos and human amniotic fluid (6). It is secreted by osteoblasts and inhibits monocyte differentiation into osteoclasts, thereby contributing to cortical bone thickness and bone mineral density [1279, 7]. Wnt-16 is over-expressed in cells undergoing replicative senescence, and it is up-regulated in articular cartilage by injury and osteoarthritis (9, 10). Wnt-16b expression in skin is up-regulated and enhances cell survival in human basal cell carcinomas (11). Its expression is also up-regulated by DNA damage (radiation and chemotherapy) in stroma surrounding prostate tumors, causing enhanced survival and treatment resistance in the tumor cells (12). Pre-B acute lymphoblastic leukemia with t(1;19) translocation, creating an E2A-Pbx1 fusion protein, also causes up-regulation of Wnt-16 that confers resistance to apoptosis (13, 14). Wnt-16 signaling through both canonical and JNK-mediated (non-canonical) pathways is reported (9-11).

References

  1. Clevers, H. and R. Nusse (2012) Cell 149:1192.
  2. Katoh, H. and M. Katoh (2005) Oncol. Rep. 13:771.
  3. Fear, M.W. et al. (2000) Biochem. Biophys. Res. Commun. 278:814.
  4. Hayashi, K. et al. (2009) Biol. Reprod. 80:989.
  5. Corrigan, P.M. et al. (2009) Stem Cells Dev. 18:759.
  6. Nath, A.K. et al. (2009) PLoS One 4:e4221.
  7. Zheng, H.F. et al. (2012) PLoS Genet. 8:31002745.
  8. Moverare-Skrtic, S. et al. (2014) Nat. Med. 20:1279.
  9. Dell'accio, F. et al. (2008) Arthritis Rheum. 58:1410.
  10. Binet, R. et al. (2009) Cancer Res. 69:9183.
  11. Teh, M.T. et al. (2006) J. Cell Sci. 120:330.
  12. Sun, Y. et al. (2012) Nat. Med. 18:1359.
  13. McWhirter, J.R. et al. (1999) Proc. Natl. Acad. Sci. USA 96:11464.
  14. Mazieres, J. et al. (2005) Oncogene 24:5396.

Long Name

Wingless-type MMTV Integration Site Family, Member 16B

Alternate Names

WNT16, Wnt16b

Entrez Gene IDs

51384 (Human); 93735 (Mouse); 500047 (Rat)

Gene Symbol

WNT16

UniProt

Q9QYS1

Additional Wnt-16b Products

Product Documents for Recombinant Mouse Wnt-16b Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 9148-WN (with carrier)
Download SDS 9148-WN/CF (carrier free)

Product Specific Notices for Recombinant Mouse Wnt-16b Protein

For research use only

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