Recombinant Mouse Wnt-9b Protein

R&D Systems, part of Bio-Techne | Catalog # 3669-WN

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Carrier Free
3669-WN-025/CF
Catalog #
Availability
Size / Price
Qty
With Carrier
3669-WN-025
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

CHO

Accession #

O35468

Conjugate

Unconjugated

Applications

Bioactivity

View all Wnt-9b Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-9b protein
Ala24-Arg359

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala24

Predicted Molecular Mass

36.8 kDa

SDS-PAGE

39-41 kDa, reducing conditions

Activity

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells.
The typical ED50 range is 100-400 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 3669-WN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
Carrier Free: 3669-WN/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

Background: Wnt-9b

Mouse Wnt-9b, previously called Wnt-14b or Wnt-15, is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved, cysteine-rich secreted glycoproteins important for normal developmental processes (1-3). Wnts mainly transduce signals by binding to receptors of the Frizzled family, in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6) (1, 2). The 359 aa mouse Wnt-9b precursor contains a 336 aa mature region with 24 conserved cysteines (3). Mature mouse Wnt-9b shares 93%, 98%, 92%, 92% and 91% aa identity with human, rat, canine, equine and bovine Wnt-9b, respectively. It is evolutionarily related to Wnt-9a and Wnt-3, which share 63% and 32% aa identity, respectively (4). Wnt-9b mRNA is expressed in late embryos and in adult kidney, with lesser amounts in brain and liver (3, 4). It appears to direct mesenchymal-to-epithelial transition in the kidney and other tissues (5). During kidney development, it is expressed in the ureteric bud and induces mesonephric and metanephric tubulogenesis, nephron development, and caudal extension of the Mullerian duct, acting upstream of Wnt-4 (5-7). Induction is dependent on beta-catenin activity, implicating the canonical signaling pathway (7). Mice devoid of Wnt-9b die shortly after birth due to kidney agenesis, while low expression results in small kidneys with fewer nephrons (1, 5, 6). Mutations of Wnt-9b also cause incompletely penetrant cleft lip and palate in mice, indicating its involvement with facial midline morphogenesis (8, 9). It has weak transforming activity compared to other transforming Wnts (4).

References

  1. www.stanford.edu/~rnusse/wntwindow.html
  2. Logan, C. Y. and R. Nusse (2004) Annu. Rev. Cell Dev. Biol. 20:781.
  3. Kirikoshi, H. and  M. Katoh (2002) Int. J. Mol. Med. 9:135.
  4. Qian, J. et al. (2003) Genomics 81:34.
  5. Carroll, T. J. et al. (2005) Dev. Cell 9:283.
  6. Merkel, C. E. et al. (2007) Pediatr. Nephrol. 22:1825.
  7. Park, J-S. et al. (2007) Development 134:2533.
  8. Lan, Y. et al. (2006) Dev. Dyn. 235:1448.
  9. Juriloff, D. M. and  M. J. Harris (2008) Birth Defects Res. A Clin. Mol. Teratol. 82:63.

Long Name

Wingless-type MMTV Integration Site Family, Member 9b

Alternate Names

Wnt-14b, Wnt-15, Wnt9b

Entrez Gene IDs

7484 (Human); 22412 (Mouse); 303586 (Rat)

Gene Symbol

WNT9B

UniProt

O35468 (Mouse)

Additional Wnt-9b Products

Product Documents for Recombinant Mouse Wnt-9b Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 3669-WN (with carrier)
Download SDS 3669-WN/CF (carrier free)

Product Specific Notices for Recombinant Mouse Wnt-9b Protein

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.