Recombinant Mouse XCL1/Lymphotactin Protein
R&D Systems, part of Bio-Techne | Catalog # 486-LT
Discontinued Product
486-LT has been discontinued.
View all XCL1/Lymphotactin products.
Key Product Details
Product Specifications
Source
E. coli-derived mouse XCL1/Lymphotactin protein
Val22-Gly114, with an N-terminal Met
Val22-Gly114, with an N-terminal Met
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
10 kDa
Activity
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human XCR1.
The ED50 for this effect is 0.05-0.1 µg/mL.
The ED50 for this effect is 0.05-0.1 µg/mL.
Formulation, Preparation and Storage
486-LT
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: XCL1/Lymphotactin
Mouse lymphotactin (Lptn) and its human homologue (also named human SCM-1 and ATAC) belong to the C or gamma subfamily of chemokines. The C chemokines lack two (the 1st and 3rd) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Mouse lymphotactin encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of lymphotactin is restricted to activated mouse pro-T cells and to activated, class I MHC restricted T cells. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse. Recombinant mouse lymphotactin has been shown to have chemotactic activity for lymphocytes, NK cells and mouse splenocytes.
References
- Kelner, G. et al. (1994) Science 266:1395.
- Hedrick, J.A. et al. (1997) J. Immunol. 158:1533.
Long Name
Chemokine [C Motif] Ligand 1
Alternate Names
ATAC, Lymphotactin, SCM-1 alpha
Gene Symbol
XCL1
UniProt
Additional XCL1/Lymphotactin Products
Product Documents for Recombinant Mouse XCL1/Lymphotactin Protein
Product Specific Notices for Recombinant Mouse XCL1/Lymphotactin Protein
For research use only
Loading...
Loading...
Loading...