Recombinant Rat CD155/PVR Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9744-CD

R&D Systems, part of Bio-Techne
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9744-CD-050
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Key Product Details

Source

HEK293

Accession #

NP_058772

Conjugate

Unconjugated

Applications

Bioactivity

View all CD155/PVR Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived rat CD155/PVR protein
Rat CD155/PVR
(Glu34-Leu351)
Accession # NP_058772		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu34

Predicted Molecular Mass

61 kDa

SDS-PAGE

92-108 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse CD96 (Catalog # 5690-CD) is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Rat CD155/PVR Fc Chimera that produces 50% optimal binding response is 1-8 ng/mL.

Scientific Data Images for Recombinant Rat CD155/PVR Fc Chimera Protein, CF

Recombinant Rat CD155/PVR Fc Chimera Protein Binding Activity

Recombinant Rat CD155/PVR Fc Chimera Protein Binding Activity

When Recombinant Mouse CD96 (Catalog # 5690-CD) is coated at 1 µg/mL, 100 µL/well, Recombinant Rat CD155/PVR Fc Chimera (Catalog # 9744-CD) binds with an ED50 of 1-8 ng/mL.

Formulation, Preparation and Storage

9744-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD155/PVR

CD155, also known as PVR (poliovirus receptor), Necl-5 (nectin-like molecule-5) and, in rodents, TAGE4 (tumor-associated glycoprotein E4), is a 70-kDa type I transmembrane glycoprotein in the nectin-related family of adhesion proteins within the immunoglobulin superfamily (1, 2). CD155 may play a role in cancer cell invasion and migration, and binds other molecules including Vitronectin, Nectin-3, DNAM-1/CD226, CD96, and TIGIT but does not bind homophilicly (3, 4). Mature rat CD155 consists of three Ig-like domains and a 21 amino acid (aa) transmembrane segment. Within the ECD, rat CD155 shares 44% and 73% aa sequence identity with human and mouse CD155, respectively. CD155 is up-regulated on endothelial cells by IFN-gamma and is highly expressed on immature thymocytes, lymph node dendritic cells, and tumor cells of epithelial and neuronal origin (1, 2, 5-8). It is preferentially expressed on Th17 cells compared to Th2 cells (9), and its activation promotes the development of Th1 responses (10). On migrating cells, CD155 is concentrated at the leading edge, where it binds basement membrane Vitronectin, recruits Nectin-3-expressing cells, and cooperates with PDGF and Integrin alpha v beta 3 to promote cell migration (1, 3, 11). Enhanced CD155 expression in tumor cells contributes to loss of contact inhibition and increased migration but also allows tumor cell recognition and killing by DNAM-1 or CD96 expressing NK cells (1, 6, 12). Binding of monocyte DNAM-1 to endothelial cell CD155 promotes transendothelial migration (7).

References

  1. Mandai, K. et al. (2015) Curr. Top. Dev. Biol. 112:197.
  2. Ravens, I. et al. (2003) Biochem. Biophys. Res. Commun. 312:1364.
  3. Sloan, K. et al. (2004) BMC Cancer. 4:73.
  4. Sato, T. et al. (2004) Genes to Cells 9:791.
  5. Escalante, N.K. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:1177.
  6. Xu, Z. and B. Jin (2010) Cell. Mol. Immunol. 7:11.
  7. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
  8. Maier, M.K. et al. (2007) Eur. J. Immunol. 37:2214.
  9. Lozano, E. et al. (2013) J. Immunol. 191:3673.
  10. Yamashita-Kanemaru, Y. et al. (2015) J. Immunol. 194:5644.
  11. Mueller, S. and E. Wimmer (2003) J. Biol. Chem. 278:31251.
  12. Chan, C.J. et al. (2010) J. Immunol. 184:902.

Long Name

Poliovirus Receptor

Alternate Names

CD155, HVED, Necl-5, PVR, PVS

Entrez Gene IDs

5817 (Human); 52118 (Mouse); 25066 (Rat); 102136444 (Cynomolgus Monkey); 712851 (Rhesus Macaque)

Gene Symbol

PVR

UniProt

NP_058772

Additional CD155/PVR Products

Product Documents for Recombinant Rat CD155/PVR Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Rat CD155/PVR Fc Chimera Protein, CF

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