Recombinant Rat MANF His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10430-MN

R&D Systems, part of Bio-Techne
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10430-MN-050
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Key Product Details

Source

NS0

Accession #

P0C5H9.1

Conjugate

Unconjugated

Applications

Bioactivity

View all MANF Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat MANF protein
Leu22-Leu179, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu22

Predicted Molecular Mass

19 kDa

SDS-PAGE

18-23 kDa, under reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Rat MANF, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.

Scientific Data Images for Recombinant Rat MANF His-tag Protein, CF

Recombinant Rat MANF His-tag Protein SDS-PAGE

Recombinant Rat MANF His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Rat MANF His-tag Protein (Catalog # 10430-MN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-23 kDa and 13-18 kDa, respectively.

Formulation, Preparation and Storage

10430-MN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MANF

Mesencephalic astrocyte-derived neurotrophic factor (MANF), also known as arginine-rich, mutated in early stage tumors (ARMET) and arginine-rich protein (ARP), is a 20 kDa member of the ARMET family of proteins (1). The name ARMET comes from the fact that the protein was initially thought to be 50 amino acid (aa) longer at the N-terminus and to contain an arginine-rich region (2-5). The presence of a specific mutation changing the previously numbered codon 50 from ATG to AGG, or deletion of that codon, has been reported in a variety of solid tumors (2-4). Rat MANF is synthesized as a 179 aa precursor that contains a 21 aa signal sequence and a 158 aa mature chain. Mature rat MANF is 99% identical to mature human and mouse MANF. MANF is localized to the endoplasmic reticulum (ER) and Golgi apparatus, and is also secreted (5). In the CNS, MANF selectively protects nigral dopaminergic neurons, versus GABAergic or serotonergic neurons, which suggests that MANF may be indicated for the treatment of Parkinson's disease (1). MANF is also one of the 12 commonly unfolded protein response (UPR)-up-regulated genes (5). One study showed that MANF plays an important role in protecting cells against tunicamycin and thapsigargin-induced cell death (5). Loss of MANF renders cells more susceptible to those drugs, but also increases cell proliferation and decreases cell size (5). Another study showed that MANF is an endoplasmic reticulum stress response (ERSR) gene in the heart that can be induced and secreted in response to ER stresses, including ischemia, and that extracellular MANF may protect cardiac myocytes in an autocrine and paracrine manner (6).

References

  1. Petrova, P.S. et al. (2003) J. Mol. Neurosci. 20:173.
  2. Shridhar, V. et al. (1996) Oncogene 12:1931.
  3. Shridhar, R. et al. (1996) Cancer Res. 56:5576.
  4. Shridhar, V. et al. (1997) Oncogene 14:2213.
  5. Apostolou, A. et al. (2008) Exp. Cell Res. 314:2454.
  6. Tadimalla, A. et al. (2008) Circ. Res. 103:1249.

Long Name

Mesencephalic Astrocyte-derived Neurotrphic Factor

Alternate Names

ARMET, ARP

Entrez Gene IDs

7873 (Human); 74840 (Mouse); 315989 (Rat)

Gene Symbol

MANF

UniProt

P0C5H9.1

Additional MANF Products

Product Documents for Recombinant Rat MANF His-tag Protein, CF

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Product Specific Notices for Recombinant Rat MANF His-tag Protein, CF

For research use only

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