Recombinant SARS-CoV-2 Spike S2 Subunit His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10584-CV

R&D Systems, part of Bio-Techne
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10584-CV-01M
10584-CV-100
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Key Product Details

Source

T. ni (baculovirus)

Accession #

YP_009724390.1

Conjugate

Unconjugated

Applications

Bioactivity

View all Spike S2 Subunit Proteins and Enzymes »

Product Specifications

Source

Trichoplusia ni, T. ni (baculovirus)-derived sars-cov-2 Spike S2 Subunit protein
Ser686-Lys1211, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser686

Predicted Molecular Mass

60 kDa

SDS-PAGE

65-75 kDa, under reducing conditions

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant SARS-CoV-2 Spike S2 Subunit His-tag Protein, CF

Recombinant SARS-CoV-2 Spike S2 Subunit His-tag Protein SDS-PAGE

Recombinant SARS-CoV-2 Spike S2 Subunit His-tag Protein SDS-PAGE

2 μg/lane of Recombinant SARS-CoV-2 Spike S2 Subunit His-tag (Catalog # 10584-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-75 kDa.

Formulation, Preparation and Storage

10584-CV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Spike S2 Subunit

SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that also include MERS and SARS-CoV-1. Coronaviruses are commonly comprised of four structural proteins: Spike protein(S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N) (1). SARS-CoV-2 Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 (2). As with most coronaviruses, proteolytic cleavage of the S protein into two distinct peptides, S1 and S2 subunits, is required for activation. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (2-4). A metallopeptidase, angiotensin-converting enzyme 2 (ACE-2), has been identified as a functional receptor for SARS-CoV2, similar to SARS-CoV-1, through interaction with a receptor binding domain (RBD) located at the C-terminus of S1 subunit (5, 6). The S2 subunit of SARS-CoV-2 shares 90% and 41% amino acid sequence identity with the S2 subunit of SARS-CoV-1 and MERS, respectively. It has been demonstrated the S Protein can invade host cells through the CD147/EMMPRIN receptor and mediate membrane fusion (7, 8).

References

  1. Rota, P.A. et al. (2003) Science 300:1394.
  2. Bosch, B.J. et al. (2003). J. Virol. 77:8801.
  3. Belouzard, S. et al. (2009) Proc. Natl. Acad. Sci. USA 106:5871.
  4. Millet, J.K. and G. R. Whittaker (2015) Virus Res. 202:120.
  5. Li, W. et al. (2003) Nature 426:450.
  6. Wong, S.K. et al. (2004) J. Biol. Chem. 279:3197.
  7. Wang, X. et al. (2020) https://doi.org/10.1038/s41423-020-0424-9.
  8. Wang, K. et al. (2020) bioRxiv https://www.biorxiv.org/content/10.1101/2020.03.14.988345v1.

Long Name

Spike Protein, S2 Subunit

Alternate Names

COVID-19, S2 protein, SARS-CoV-2, SARS-CoV-2 Spike, Spike glycoprotein Subunit 2, spike protein, Spike protein S2, structural protein, surface glycoprotein

UniProt

YP_009724390.1

Additional Spike S2 Subunit Products

Product Documents for Recombinant SARS-CoV-2 Spike S2 Subunit His-tag Protein, CF

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Product Specific Notices for Recombinant SARS-CoV-2 Spike S2 Subunit His-tag Protein, CF

For research use only

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