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Recombinant E. coli Ribonuclease Inhibitor His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72417

Novus Biologicals, part of Bio-Techne
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NBP1-72417-0.5mg
NBP1-72417-100ug

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-155 of E.coli Ribonuclease Inhibitor

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMLKQVEI FTDGSCLGNP GPGGYGAILR YRGREKTFSA GYTRTTNNRM ELMAAIVALE ALKEHCEVIL STDSQYVRQG ITQWIHNWKK RGWKTADKKP VKNVDLWQRL DAALGQHQIK WEWVKGHAGH PENERCDELA RAAAMNPTLE DTGYQVEV

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli Ribonuclease Inhibitor His Protein

SDS-PAGE: Recombinant E. coli Ribonuclease Inhibitor His Protein [NBP1-72417]

SDS-PAGE: Recombinant E. coli Ribonuclease Inhibitor His Protein [NBP1-72417]

SDS-Page: Recombinant E. coli Ribonuclease Inhibitor Protein [NBP1-72417] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-72417
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2 mM DTT
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ribonuclease Inhibitor

rnhA is an endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Localized to the nucleus, this protein mediates the removal of Okazaki fragment RNA primers that are present on the lagging strand during DNA replication. rnhA catalyzes the endonucleolytic cleavage of RNA to a 5'-phosphomonoester and is able to bind magnesium or manganese as cofactors. Recombinant e.coli rnhA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

Placental ribonuclease inhibitor, Placental RNase inhibitor, PRI, RAIMGC4569, ribonuclease inhibitor, ribonuclease/angiogenin inhibitor, ribonuclease/angiogenin inhibitor 1MGC18200, RNHMGC54054

Gene Symbol

RNH1

Additional Ribonuclease Inhibitor Products

Product Documents for Recombinant E. coli Ribonuclease Inhibitor His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant E. coli Ribonuclease Inhibitor His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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