RIPK3/RIP3 Antibody Blocking Peptide
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77299PEP
Key Product Details
Source
Synthetic
Conjugate
Unconjugated
Applications
Antibody Competition, Western Blot
Product Specifications
Description
A blocking peptide containing 14 amino acids of mouse RIP3.
Source: Synthetic
(Accession #: AAF03133)
Purity
N/A
Application Notes
This peptide is useful as a blocking peptide for NBP1-77299. For further blocking peptide related protocol, click here.
Protein / Peptide Type
Antibody Blocking Peptide
Scientific Data Images for RIPK3/RIP3 Antibody Blocking Peptide
Western Blot: RIPK3/RIP3 Antibody Blocking Peptide [NBP1-77299PEP]
Western Blot: RIPK3/RIP3 Antibody Blocking Peptide [NBP1-77299PEP] - Western blot analysis of RIP3 in mouse 3T3 whole cell lysate in the absence (A) or presence (B) of RIPK3/RIP3 Antibody blocking peptide [NBP1-77299PEP] with RIP3 antibody at 1 ug/mL. TMW 53 kda, observed molecular weight ~60 kda.Formulation, Preparation and Storage
NBP1-77299PEP
Formulation | PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin. |
Preservative | 0.02% Sodium Azide |
Concentration | 0.2 mg/ml |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Store at -20C. Avoid freeze-thaw cycles. |
Background: RIPK3/RIP3
RIPK3 has several phosphorylation sites that are required for its role in necroptosis. For example, serine204 in mouse, which is conserved in the human (serine199), is necessary for necroptosis while serine residues 232 and 227 are both required for RIPK3s interaction with MLKL (2). The core necroptosis proteins RIPK1/RIPK3 and MLKL are implicated in several disease states such as neurodegeneration, cardiovascular, hepatic and pulmonary disease (3).
References
1. Orozco, S., & Oberst, A. (2017). RIPK3 in cell death and inflammation: the good, the bad, and the ugly. Immunological Reviews. https://doi.org/10.1111/imr.12536
2. Dhuriya, Y. K., & Sharma, D. (2018). Necroptosis: A regulated inflammatory mode of cell death. Journal of Neuroinflammation. https://doi.org/10.1186/s12974-018-1235-0
3. Choi, M. E., Price, D. R., Ryter, S. W., & Choi, A. M. K. (2019). Necroptosis: A crucial pathogenic mediator of human disease. JCI Insight. https://doi.org/10.1172/jci.insight.128834
4. Lee, K.-H., & Kang, T.-B. (2019). The Molecular Links between Cell Death and Inflammasome. Cells. https://doi.org/10.3390/cells8091057
Long Name
Receptor (TNFRSF)-Interacting Serine-Threonine Kinase 3
Alternate Names
RIP3
Gene Symbol
RIPK3
Additional RIPK3/RIP3 Products
Product Documents for RIPK3/RIP3 Antibody Blocking Peptide
Product Specific Notices for RIPK3/RIP3 Antibody Blocking Peptide
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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