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RIPK3/RIP3 Antibody Blocking Peptide

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77299PEP

Novus Biologicals, part of Bio-Techne
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NBP1-77299PEP

Key Product Details

Source

Synthetic

Conjugate

Unconjugated

Applications

Antibody Competition, Western Blot

Product Specifications

Description

A blocking peptide containing 14 amino acids of mouse RIP3.

Source: Synthetic

(Accession #: AAF03133)

Purity

N/A

Application Notes

This peptide is useful as a blocking peptide for NBP1-77299. For further blocking peptide related protocol, click here.

Protein / Peptide Type

Antibody Blocking Peptide

Scientific Data Images for RIPK3/RIP3 Antibody Blocking Peptide

Western Blot: RIPK3/RIP3 Antibody Blocking Peptide [NBP1-77299PEP]

Western Blot: RIPK3/RIP3 Antibody Blocking Peptide [NBP1-77299PEP]

Western Blot: RIPK3/RIP3 Antibody Blocking Peptide [NBP1-77299PEP] - Western blot analysis of RIP3 in mouse 3T3 whole cell lysate in the absence (A) or presence (B) of RIPK3/RIP3 Antibody blocking peptide [NBP1-77299PEP] with RIP3 antibody at 1 ug/mL. TMW 53 kda, observed molecular weight ~60 kda.

Formulation, Preparation and Storage

NBP1-77299PEP
Formulation PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin.
Preservative 0.02% Sodium Azide
Concentration 0.2 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: RIPK3/RIP3

RIPK3, Receptor interacting serine/threonine kinase 3 or RIP-like protein kinase 3 (human RIPK3 isoform1 theoretical molecular weight 57kDa) is a cytosolic protein with an amino terminal active kinase domain. RIP kinases, RIPK1 and RIPK3 play a central role in the induction of necroptosis, a form of programmed and inflammatory cell death that is caspase-independent (1). Necroptosis is a form of programmed necrosis which is initiated through the activation of RIPK3 by various ligands such as Fas, LPS and TNF. Formation of the necrosome results from the interaction between RIPK1 and RIPK3 mediated through their RIP homotypic interaction motifs (RHIMs) (1, 2). Following necrosome formation, RIPK3 activation leads to the phosphorylation of mixed lineage kinase domain-like protein (MLKL) which induces its oligomerization and translocation to the cell membrane where it alters plasma membrane integrity (2). Loss of membrane integrity results in lytic cell death, release of damage associated molecular patterns (DAMPs) and inflammation (2, 3). Therefore, the interaction of RIPK3 with other RHIM proteins is necessary for the initiation of necroptosis, while the execution phase is dependent on the activation of MLKL (4).

RIPK3 has several phosphorylation sites that are required for its role in necroptosis. For example, serine204 in mouse, which is conserved in the human (serine199), is necessary for necroptosis while serine residues 232 and 227 are both required for RIPK3s interaction with MLKL (2). The core necroptosis proteins RIPK1/RIPK3 and MLKL are implicated in several disease states such as neurodegeneration, cardiovascular, hepatic and pulmonary disease (3).

References

1. Orozco, S., & Oberst, A. (2017). RIPK3 in cell death and inflammation: the good, the bad, and the ugly. Immunological Reviews. https://doi.org/10.1111/imr.12536

2. Dhuriya, Y. K., & Sharma, D. (2018). Necroptosis: A regulated inflammatory mode of cell death. Journal of Neuroinflammation. https://doi.org/10.1186/s12974-018-1235-0

3. Choi, M. E., Price, D. R., Ryter, S. W., & Choi, A. M. K. (2019). Necroptosis: A crucial pathogenic mediator of human disease. JCI Insight. https://doi.org/10.1172/jci.insight.128834

4. Lee, K.-H., & Kang, T.-B. (2019). The Molecular Links between Cell Death and Inflammasome. Cells. https://doi.org/10.3390/cells8091057

Long Name

Receptor (TNFRSF)-Interacting Serine-Threonine Kinase 3

Alternate Names

RIP3

Gene Symbol

RIPK3

Additional RIPK3/RIP3 Products

Product Documents for RIPK3/RIP3 Antibody Blocking Peptide

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RIPK3/RIP3 Antibody Blocking Peptide

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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