Recombinant Human RNASEH2A His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23414

Novus Biologicals, part of Bio-Techne
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NBP2-23414
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all RNASEH2A Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-299 of Human RNASEH2A

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMDLSELE RDNTGRCRLS SPVPAVCRKE PCVLGVDEAG RGPVLGPMVY AICYCPLPRL ADLEALKVAD SKTLLESERE RLFAKMEDTD FVGWALDVLS PNLISTSMLG RVKYNLNSLS HDTATGLIQY ALDQGVNVTQ VFVDTVGMPE TYQARLQQSF PGIEVTVKAK ADALYPVVSA ASICAKVARD QAVKKWQFVE KLQDLDTDYG SGYPNDPKTK AWLKEHVEPV FGFPQFVRFS WRTAQTILEK EAEDVIWEDS ASENQEGLRK ITSYFLNEGS QARPRSSHRY FLERGLESAT SL

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

35.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human RNASEH2A His Protein

SDS-PAGE: Recombinant Human RNASEH2A His Protein [NBP2-23414]

SDS-PAGE: Recombinant Human RNASEH2A His Protein [NBP2-23414]

SDS-Page: RNASEH2A Protein [NBP2-23414]

Formulation, Preparation and Storage

NBP2-23414
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RNASEH2A

Ribonuclease H2 subunit A, also known as RNASEH2A, belongs to the RNase HII family and eukaryotic subfamily. Localized to the nucleus, RNASEH2A mediates the removal of Okazaki fragment RNA primers that are present on the lagging strand during DNA replication. RNASEH2A catalyzes the endonucleolytic cleavage of RNA to a 5'-phosphomonoester and is able to bind magnesium or manganese as cofactors. Defects in RNASEH2A are the cause of Aicardi-Goutieres syndrome type 4 (AGS4). Recombinant human RNASEH2A protein, fused to His-tag at N-terminus, was expressed in E.coli

Alternate Names

AGS4RNase H2 subunit A, Aicardi-Goutieres syndrome 4, Aicardi-Goutieres syndrome 4 protein, JUNB, ribonuclease H2 subunit A, ribonuclease H2, large subunit, ribonuclease H2, subunit A, Ribonuclease HI large subunit, Ribonuclease HI subunit A, ribonuclease HI, large subunit, RNase H(35), RNASEHIEC 3.1.26.4, RNHIARNase HI large subunit, RNHL

Gene Symbol

RNASEH2A

Additional RNASEH2A Products

Product Documents for Recombinant Human RNASEH2A His Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human RNASEH2A His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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