Recombinant Human RPA2 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-50878

Novus Biologicals, part of Bio-Techne
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NBP1-50878-0.25mg
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all RPA2 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-270 of Human RPA2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMWNSGFE SYGSSSYGGA GGYTQSPGGF GSPAPSQAEK KSRARAQHIV PCTISQLLSA TLVDEVFRIG NVEISQVTIV GIIRHAEKAP TNIVYKIDDM TAAPMDVRQW VDTDDTSSEN TVVPPETYVK VAGHLRSFQN KKSLVAFKIM PLEDMNEFTT HILEVINAHM VLSKANSQPS AGRAPISNPG MSEAGNFGGN SFMPANGLTV AQNQVLNLIK ACPRPEGLNF QDLKNQLKHM SVSSIKQAVD FLSNEGHIYS TVDDDHFKST DAE

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

31.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human RPA2 His Protein

SDS-PAGE: Recombinant Human RPA2 His Protein [NBP1-50878]

SDS-PAGE: Recombinant Human RPA2 His Protein [NBP1-50878]

SDS-Page: Recombinant Human RPA2 Protein [NBP1-50878] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain, 31.7 kDa (293aa) confirmed by MALDI-TOF

Formulation, Preparation and Storage

NBP1-50878
Formulation 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl, 2mM DTT
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPA2

Replication Protein A (RPA) is a single stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 14, 32 and 70kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. The C-terminus of RPA2 can specfically intereact with the DNA repair enzyme UNG2 and repair factors XPA and Rad52, each of which functions in a different repair pathway. In addition, this protein binds specifically to the SH2 domain of Stat3 in vivo, and overexpression of RPA2 corresponds to the augmented growth factor-stimulated tyrosine phosphorylation and transcription activities of Stat3. Recombinant human RPA2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Replication Protein A2, 32kDa

Alternate Names

REPA2, RPA32

Gene Symbol

RPA2

Additional RPA2 Products

Product Documents for Recombinant Human RPA2 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human RPA2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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