Recombinant Saccharomyces cerevisiae GLC8 His Protein

Name: Recombinant Saccharomyces cerevisiae GLC8 His Protein
Brand: Novus Biologicals
SKU: NBC1-18408

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-18408

Submit a review

Product Datasheet / COA

100% Guarantee

Technical Data
Citations & Reviews

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Saccharomyces cerevisiae GLC8 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-229 of Saccharomyces cerevisiae GLC8

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMGGI LKNPLALSPE QLAQQDPETL EEFRRQVYEN TQKNAKLTSH KRNIPGLDNT KEEGEIIGTS STFLPKDTLS LKHEQDMLAK MTPEERVQWN QRNLAENEIT KKQFQDIHID EPKTPYQGAV DPHGEYYRVD DDEDEDNSDK KPCQVANDDI DDLSLGEPEF EIKENKQPDF ETNDENDEDS PEARHKKFEE MRKKHYDVRA IFNKKSREAL KDEDEDEDDS TTKEP

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

30.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Saccharomyces cerevisiae GLC8 His Protein

SDS-PAGE: Recombinant Saccharomyces cerevisiae GLC8 His Protein [NBC1-18408]

SDS-Page: GLC8 Protein [NBC1-18408] - GLC8, 30.7kDa (265aa), confirmed by MALDI-TOF with a purity of 95% by SDS PAGE

Formulation, Preparation and Storage

NBC1-18408

Formulation	20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Saccharomyces cerevisiae GLC8

GLC8 is a regulatory subunit of protein phosphatase 1 (Glc7p). This protein involved in glycogen metabolism and chromosome segregation, proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2. Under normal conditions, Glc8p activates Glc7p, but when Glc8p is over produced, it may also inhibit Glc7p function. Glc8p is activated upon phosphoylation by Pho85p complexed with four cyclins (Pcl6p, Pcl7p, Pcl8p, or Pcl10p). Recombinant yeast GLC8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

Glc8p

Gene Symbol

GLC8

Additional Saccharomyces cerevisiae GLC8 Products

Product Documents for Recombinant Saccharomyces cerevisiae GLC8 His Protein

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for Recombinant Saccharomyces cerevisiae GLC8 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations
Reviews