Recombinant Human SAP18 His Protein

Name: Recombinant Human SAP18 His Protein
Brand: Novus Biologicals
SKU: NBC1-28776

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-28776

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Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all SAP18 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 20- 172 of Human SAP18

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAVESRVTQE EIKKEPEKPI DREKTCPLLL RVFTTNNGRH HRMDEFSRGN VPSSELQIYT WMDATLKELT SLVKEVYPEA RKKGTHFNFA IVFTDVKRPG YRVKEIGSTM SGRKGTDDSM TLQSQKFQIG DYLDIAITPP NRAPPPSGRM RPY

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

19.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human SAP18 His Protein

SDS-PAGE: Recombinant Human SAP18 His Protein [NBC1-28776]

SDS-Page: Recombinant Human SAP18 Protein [NBC1-28776] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBC1-28776

Formulation	20 mM Tris-HCl buffer (pH8.0), 0.1 M NaCl, 30% glycerol, 1 mM DTT
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SAP18

SAP18, also known as Sin3A-associated protein, is component of the histone deacetylase complex that plays an important role in the regulation of eukaryotic gene expression. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter. It also has been shown to play a key role in gene-specific recruitment of the HDAC complex by a number of transcription factors including Gli, GAGA, and Bicoid. Recombinant SAP18 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

2HOR0202cell growth inhibiting protein 38, Cell growth-inhibiting gene 38 protein, cell growth-inhibiting protein 38, histone deacetlyase complex subunit SAP18, histone deacetylase complex subunit SAP18,18 kDa Sin3-associated polypeptide, MGC27131, SAP18P, Sin3A-associated protein, 18kDa, Sin3-associated polypeptide p18, sin3-associated polypeptide, 18 kDa, sin3-associated polypeptide, p18

Gene Symbol

SAP18

Additional SAP18 Products

Product Documents for Recombinant Human SAP18 His Protein

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COA

Product Specific Notices for Recombinant Human SAP18 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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