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Recombinant Human SAR1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30177

Novus Biologicals, part of Bio-Techne
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NBP1-30177

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1- 198 of Human SAR1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSFIFEWIYN GFSSVLQFLG LYKKSGKLVF LGLDNAGKTT LLHMLKDDRL GQHVPTLHPT SEELTIAGMT FTTFDLGGHE QARRVWKNYL PAINGIVFLV DCADHSRLVE SKVELNALMT DETISNVPIL ILGNKIDRTD AISEEKLREI FGLYGQTTGK GNVTLKELNA RPMEVFMCSV LKRQGYGEGF RWLSQYID

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

24.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human SAR1 His Protein

SDS-PAGE: Recombinant Human SAR1 His Protein [NBP1-30177]

SDS-PAGE: Recombinant Human SAR1 His Protein [NBP1-30177]

SDS-Page: Recombinant Human SAR1 His Protein [NBP1-30177] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-30177
Formulation 20 mM Tris-HCl buffer (pH8.0), 1 mM DTT, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SAR1

SAR1a gene homolog 1, also known as SAR1A, belongs to the small GTPase superfamily. Sar1 is an essential component of COPII vesicle coats involved in export of cargo from the ER. The GTPase activity of Sar1 functions as a molecular switch to control protein-protein and protein-lipid interactions that direct vesicle budding from the ER. GDP-bound Sar1 interacts with the membrane-bound exchange factor Sec12 and exchanges its bound GDP for GTP. Recombinant human SAR1A, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

COPII-associated small GTPase, EC 3.6.5, masra2, SAR1 gene homolog A, SAR1 homolog A (S. cerevisiae), SAR1a gene homolog (S. cerevisiae) 1, SAR1a gene homolog 1, SAR1a gene homolog 1 (S. cerevisiae), SAR1SARA1GTP-binding protein SAR1a, SARA

Gene Symbol

SAR1A

Additional SAR1 Products

Product Documents for Recombinant Human SAR1 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human SAR1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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