Recombinant Human SDNSF/MCFD2 T7 Protein

Name: Recombinant Human SDNSF/MCFD2 T7 Protein
Brand: Novus Biologicals
SKU: NBP1-37074

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-37074

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Technical Data
Citations & Reviews

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

SDS-PAGE

View all SDNSF/MCFD2 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal T7-tag and corresponding to the amino acids 27-146 of Human SDNSF/MCFD2

Source: E.coli

Amino Acid Sequence: MASMTGGQQM GRGSHMEEPA ASFSQPGSMG LDKNTVHDQE HIMEHLEGVI NKPEAEMSPQ ELQLHYFKMH DYDGNNLLDG LELSTAITHV HKEEGSEQAP LMSEDELINI IDGVLRDDDK NNDGYIDYAE FAKSLQ

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

15.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human SDNSF/MCFD2 T7 Protein

SDS-PAGE: Recombinant Human SDNSF/MCFD2 T7 Protein [NBP1-37074]

SDS-Page: MCFD2 Protein [NBP1-37074] - MCFD2, 15.1 kDa (136aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Formulation, Preparation and Storage

NBP1-37074

Formulation	20 mM Tris-HCl buffer (pH7.5), 100 mM NaCl, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SDNSF/MCFD2

Multiple coagulation factor deficiency 2 (MCFD2), also known as SDNSF. This is expressed by neural stem/progenitor cells of the hippocampus, and localized to region where neurogenesis persists throughout life. It has been found to prevent NSC cell death and to maintain stem cell characteristics. This protein forms a complex with LAMN1 that facilitates the transport of coagulation factors V and VIII from the endoplasmic reticulum to the Golgi apparatus via an endoplasmic reticulum Golgi intermediate compartment. Mutations in the MCFD2 gene may cause Factor V and Factor VIII combined deficiency. Recombinant human MCFD2, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Stem Cell-derived Neuronal Stem/Progenitor Cell Supporting Factor

Alternate Names

MCFD2

Gene Symbol