Recombinant Human SerpinB2 Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35202
Key Product Details
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 415 amino acids corresponding to SerpinB2 Source: E. coli
Uniprot ID: P05120
Amino Acid Sequence: MEDLCVANTL FALNLFKHLA KASPTQNLFL SPWSISSTMA MVYMGSRGST EDQMAKVLQF NEVGANAVTP MTPENFTSCG FMQQIQKGSY PDAILQAQAA DKIHSSFRSL SSAINASTGN YLLESVNKLF GEKSASFREE YIRLCQKYYS SEPQAVDFLE CAEEARKKIN SWVKTQTKGK IPNLLPEGSV DGDTRMVLVN AVYFKGKWKT PFEKKLNGLY PFRVNSAQRT PVQMMYLREK LNIGYIEDLK AQILELPYAG DVSMFLLLPD EIADVSTGLE LLESEITYDK LNKWTSKDKM AEDEVEVYIP QFKLEEHYEL RSILRSMGME DAFNKGRANF SGMSERNDLF LSEVFHQAMV DVNEEGTEAA AGTGGVMTGR TGHGGPQFVA DHPFLFLIMH KITNCILFFG RFSSP
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 0.1 EU/ug of SerpinB2 as determined by LAL method.
Predicted Molecular Mass
46.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
SerpinB2 protein is fully biologically active when compared to standard. The biologically active was determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37 degrees C. Half maximal inhibition against 1.0 ug/ml of single chain tPA was at a concentration of 1.0 ug/ml.
Protein / Peptide Type
Recombinant Protein
Formulation, Preparation and Storage
NBP2-35202
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1 mM Cysteine, with . |
| Preservative | 5% Trehalose |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: Serpin B2
Additional Serpin B2 Products
Product Documents for Recombinant Human SerpinB2 Protein
Product Specific Notices for Recombinant Human SerpinB2 Protein
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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