Recombinant Human SF20/MYDGF His Protein

Name: Recombinant Human SF20/MYDGF His Protein
Brand: Novus Biologicals
SKU: NBC1-22603

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-22603

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all SF20/MYDGF Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 33-173 of Human SF20/MYDGF.

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSEPTTVAFD VRPGGVVHSF SHNVGPGDKY TCMFTYASQG GTNEQWQMSL GTSEDHQHFT CTIWRPQGKS YLYFTQFKAE VRGAEIEYAM AYSKAAFERE SDVPLKTEEF EVTKTAVAHR PGAFKAELSK LVIVAKASRT EL

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human SF20/MYDGF His Protein

SDS-PAGE: Recombinant Human SF20/MYDGF His Protein [NBC1-22603]

SDS-Page: Recombinant Human, SF20/MYDGF His Protein [NBC1-22603] - Recombinant Human SF20/MYDGF [NBC1-22603]

Formulation, Preparation and Storage

NBC1-22603

Formulation	20 mM Tris-HCl buffer (pH 8.0), 20% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SF20/MYDGF

Myeloid-Derived Growth Factor, or MYDGF, is a Bone marrow-derived monocyte protein, and it is correlated with enhanced metabolic activity, suppression of apoptosis, and stimulation of cell proliferation (1). MYDGF is expressed predominantly in inflammatory cells, such as monocytes and macrophages (1). Up-regulation of MYDGF expression was also found during adipocyte differentiation (2). Expression of MYDGF was induced in the circulation and heart tissue after myocardial infarction. It promotes cardiac myocyte survival by stimulating endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway, and inhibits cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway (1). MYDGF was found over-expressed in approximately two-thirds of Hepatocellular Carcinoma (HCC) tissues, and its expression was significantly positively correlated with that of alpha-fetoprotein (AFP) (3). In HCC, MYDGF could regulate cell proliferation through activating Akt/mitogen-activated protein kinase pathways (3). Mouse MYDGF shares 92% amino acid sequence identity with both human and rat MYDGF. Intriguingly, virtually all homologs of MYDGF have a C-terminal putative ER retention sequence BXEL (B: Arg, His, or Lys; X: variable residue; E: Glu; L: Leu), which has the potential to retain human MYDGF and its homologs in the ER, whereas truncated MYDGF without BXEL is secreted from the cell (4). However, the functions of these different forms remain unclear.

Long Name

Stromal Cell-derived Growth Factor SF20

Alternate Names

C19orf10, Ly6elg, MYDGF

Gene Symbol

MYDGF

Additional SF20/MYDGF Products

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COA

Product Specific Notices for Recombinant Human SF20/MYDGF His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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