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Recombinant Human SIVA His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22808

Novus Biologicals, part of Bio-Techne
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NBP2-22808

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-175 of Human SIVA

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPKRSCP FADVAPLQLK VRVSQRELSR GVCAERYSQE VFEKTKRLLF LGAQAYLDHV WDEGCAVVHL PESPKPGPTG APRAARGQML IGPDGRLIRS LGQASEADPS GVASIACSSC VRAVDGKAVC GQCERALCGQ CVRTCWGCGS VACTLCGLVD CSDMYEKVLC TSCAMFET

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

21.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human SIVA His Protein

SDS-PAGE: Recombinant Human SIVA His Protein [NBP2-22808]

SDS-PAGE: Recombinant Human SIVA His Protein [NBP2-22808]

SDS-Page: SIVA Protein [NBP2-22808]

Formulation, Preparation and Storage

NBP2-22808
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 0.4M UREA
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SIVA

Apoptosis regulatory protein Siva isoform 1, also known as SIVA1, is a death domain-containing proapoptotic protein identified as an intracellular ligand of CD27 and of the glucocorticoid-induced TNFR family-related gene, which are two members of the TNFR family expressed on lymphoid cells. SIVA1 expression is up-regulated in multiple pathological processes, little is known about the signaling pathway underlying the Siva-induced apoptosis. Recombinant human SIVA1 protein, fused to His-tag at N-terminus, was expressed in E.coli. (denatured)

Alternate Names

CD27-binding (Siva) protein, CD27BPCD27-binding protein, Siva-1, SIVA1, apoptosis-inducing factor, Siva-2, SIVAapoptosis regulatory protein Siva

Gene Symbol

SIVA1

Additional SIVA Products

Product Documents for Recombinant Human SIVA His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human SIVA His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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