Recombinant Human Sorbitol Dehydrogenase His Protein

Name: Recombinant Human Sorbitol Dehydrogenase His Protein
Brand: Novus Biologicals
SKU: NBP1-44460

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44460

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all Sorbitol Dehydrogenase Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-357 of Human Sorbitol Dehydrogenase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAAAAKPNNL SLVVHGPGDL RLENYPIPEP GPNEVLLRMH SVGICGSDVH YWEYGRIGNF IVKKPMVLGH EASGTVEKVG SSVKHLKPGD RVAIEPGAPR ENDEFCKMGR YNLSPSIFFC ATPPDDGNLC RFYKHNAAFC YKLPDNVTFE EGALIEPLSV GIHACRRGGV TLGHKVLVCG AGPIGMVTLL VAKAMGAAQV VVTDLSATRL SKAKEIGADL VLQISKESPQ EIARKVEGQL GCKPEVTIEC TGAEASIQAG IYATRSGGTL VLVGLGSEMT TVPLLHAAIR EVDIKGVFRY CNTWPVAISM LASKSVNVKP LVTHRFPLEK ALEAFETFKK GLGLKIMLKC DPSDQNP

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

40.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 20,000 pmol/min/ug, and is defined as the amount of enzyme that catalyze D-fructose to D-sorbitol per minute at pH 7.5 at 37C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Sorbitol Dehydrogenase His Protein

SDS-PAGE: Recombinant Human Sorbitol Dehydrogenase His Protein [NBP1-44460]

SDS-Page: Sorbitol Dehydrogenase Protein [NBP1-44460] - SORD, 40.4 kDa (377aa) confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Formulation, Preparation and Storage

NBP1-44460

Formulation	20 mM Tris-HCl buffer (pH8.0), 0.2 M NaCl, 5mM DTT, 20% glycerol
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sorbitol Dehydrogenase

SORD, also known as L-iditol 2-dehydrogenase or SORD1, is a 357 amino acid member of the zinc-containing alcohol dehydrogenase family. It is widely expressed with highest expression in kidney and in the lens of the eye. SORD enzymatically catalyzes the zinc-dependent interconversion of polyols, such as sorbitol and xylitol, to their respective ketoses. Recombinant human SORD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

EC 1.1.1, EC 1.1.1.14, L-iditol 2-dehydrogenase, sorbitol dehydrogenase, SORD1

Gene Symbol

SORD

Additional Sorbitol Dehydrogenase Products

Product Documents for Recombinant Human Sorbitol Dehydrogenase His Protein

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download SDS-PAGE Protocol

COA

Product Specific Notices for Recombinant Human Sorbitol Dehydrogenase His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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