Recombinant Human SPOP His Protein

Name: Recombinant Human SPOP His Protein
Brand: Novus Biologicals
SKU: NBP1-51077

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51077

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Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all SPOP Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-374 of Human SPOP

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSRVPSPPPP AEMSSGPVAE SWCYTQIKVV KFSYMWTINN FSFCREEMGE VIKSSTFSSG ANDKLKWCLR VNPKGLDEES KDYLSLYLLL VSCPKSEVRA KFKFSILNAK GEETKAMESQ RAYRFVQGKD WGFKKFIRRD FLLDEANGLL PDDKLTLFCE VSVVQDSVNI SGQNTMNMVK VPECRLADEL GGLWENSRFT DCCLCVAGQE FQAHKAILAA RSPVFSAMFE HEMEESKKNR VEINDVEPEV FKEMMCFIYT GKAPNLDKMA DDLLAAADKY ALERLKVMCE DALCSNLSVE NAAEILILAD LHSADQLKTQ AVDFINYHAS DVLETSGWKS MVVSHPHLVA EAYRSLASAQ CPFLGPPRKR LKQS

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

44.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human SPOP His Protein

Western Blot: Recombinant Human SPOP His Protein [NBP1-51077]

Western Blot: Recombinant Human SPOP Protein [NBP1-51077]

Formulation, Preparation and Storage

NBP1-51077

Formulation	20 mM Tris-HCl buffer (pH 8.0), 5mM DTT, 50% glycerol, 0.2 M NaCl, 2 mM EDTA
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPOP

SPOP (speckle-type POZ protein), also known as TEF2, is a member of the Tdpoz family containing one N-terminal MATH (Meprin and TRAF homology) domain and one C-terminal BTB/POZ domain. This protein inhibits IPF1/PDX1 transactivation of established target promoters, such as insulin, may be by recruiting a repressor complex. Through an interaction with CUL-3, SPOP is involved in ubiquitinylation and protein degradation. Recombinant human SPOP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

HIB homolog 1, Roadkill homolog 1, speckle-type POZ protein, TEF2

Gene Symbol

SPOP

Additional SPOP Products

Product Documents for Recombinant Human SPOP His Protein

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COA

Product Specific Notices for Recombinant Human SPOP His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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