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Recombinant Human SSX1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22841

Novus Biologicals, part of Bio-Techne
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NBP2-22841

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-188 of Human SSX1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNGDDTF AKRPRDDAKA SEKRSKAFDD IATYFSKKEW KKMKYSEKIS YVYMKRNYKA MTKLGFKVTL PPFMCNKQAT DFQGNDFDND HNRRIQVEHP QMTFGRLHRI IPKIMPKKPA EDENDSKGVS EASGPQNDGK QLHPPGKANI SEKINKRSGP KRGKHAWTHR LRERKQLVIY EEISDPEEDD E

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

24.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human SSX1 His Protein

SDS-PAGE: Recombinant Human SSX1 His Protein [NBP2-22841]

SDS-PAGE: Recombinant Human SSX1 His Protein [NBP2-22841]

SDS-Page: SSX1 Protein [NBP2-22841]

Formulation, Preparation and Storage

NBP2-22841
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SSX1

Protein SSX1 belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. SSX proteins are localized to the nucleus and expressed in testis and several types of cancers and, therefore, they are classified as C/T (cancer/testis) antigens. These proteins may function as transcriptional repressors. SSX1 genes have been involved in the t (X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are probably responsible for transforming activity. Recombinant human SSX1 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

Cancer/testis antigen 5.1, CT5.1cancer/testis antigen family 5, member 1, MGC150425, protein SSX1, sarcoma, synovial, X-chromosome-related 1, SSRC, synovial sarcoma, X breakpoint 1MGC5162

Gene Symbol

SSX1

Additional SSX1 Products

Product Documents for Recombinant Human SSX1 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human SSX1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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