Recombinant Human Syndecan-4 Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61363
Key Product Details
Source
E. coli
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 127 amino acids corresponding to Syndecan-4. However it migrates with an apparent molecular mass of 22 kDa in SDS-PAGE Source: E. coli
Uniprot ID: P31431
Amino Acid Sequence: ESIRETEVID PQDLLEGRYF SGALPDDEDV VGPGQESDDF ELSGSGDLDD LEDSMIGPEV VHPLVPLDNH IPERAGSGSQ VPTEPKKLEE NEVIPKRISP VEESEDVSNK VSMSSTVQGS NIFERTE
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 0.1 EU/ug of Syndecan-4 as determined by LAL method.
Predicted Molecular Mass
13.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Fully biologically active when compared to standard. The specific activity is determined by binding ability in a functional ELISA. Immobilized rHuSYND4 at 500 ng/ml (100 ul/well) can bind rHubFGF with a linear range of 0.1-10 ng/ml.
Protein / Peptide Type
Recombinant Protein
Formulation, Preparation and Storage
NBP2-61363
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: Syndecan-4
Alternate Names
Amphiglycan, Ryudocan, SDC4, Syndecan4
Gene Symbol
SDC4
Additional Syndecan-4 Products
Product Documents for Recombinant Human Syndecan-4 Protein
Product Specific Notices for Recombinant Human Syndecan-4 Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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