Recombinant Human TC21/R-Ras2 His Protein

Name: Recombinant Human TC21/R-Ras2 His Protein
Brand: Novus Biologicals
SKU: NBP1-30244

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30244

Submit a review

Product Datasheet / COA

100% Guarantee

Technical Data
Citations & Reviews

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all TC21/R-Ras2 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1- 201 of Human TC21/R-Ras2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAAAGWRDGS GQEKYRLVVV GGGGVGKSAL TIQFIQSYFV TDYDPTIEDS YTKQCVIDDR AARLDILDTA GQEEFGAMRE QYMRTGEGFL LVFSVTDRGS FEEIYKFQRQ ILRVKDRDEF PMILIGNKAD LDHQRQVTQE EGQQLARQLK VTYMEASAKI RMNVDQAFHE LVRVIRKFQE QECPPSPEPT RKEKDKKGCH C

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

25.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human TC21/R-Ras2 His Protein

SDS-PAGE: Recombinant Human TC21/R-Ras2 His Protein [NBP1-30244]

SDS-Page: RRAS2 Protein [NBP1-30244] - RRAS2, 25.2 kDa (221aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Formulation, Preparation and Storage

NBP1-30244

Formulation	20 mM Tris-HCl buffer (pH8.0), 1 mM DTT, 10% glycerol, 2 mM EDTA
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TC21/R-Ras2

RAS viral (r-ras) oncogene homolog 2 isoform a, also known as RRAS2, belongs to the Ras like GTPase family that cycle between GTP-bound active and GDP-bound inactive states at the cytoplasmic face of the plasma membrane. It is implicated in the pathogenesis of human cancers. Thus, overexpression of active RRAS2 in EpH4 cells induces tumorigenicity through the phosphoinositide 3-kinase, p38 MAPK, and mTOR pathways, and these cells lose their sensitivity to the normal growth inhibitory role of TGF-beta. Recombinant human RRAS2, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Teratocarcinoma Oncogene TC21

Alternate Names

RRAS2

Gene Symbol