Recombinant TEV Protease His (N-Term) Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61441
Key Product Details
Source
E. coli
Tag
His (N-Term)
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Description
TEV protease encoded by the tobacco etch virus is a catalytic domain of the Nuclear Inclusion a (NIa) protein. It is consists of 241 a.a. amino acids with the molecular weight of 27kDa. It contains N-terminal His-tag.
Source: Escherichia coli.
One unit is defined as the amount of enzyme needed to cleave 3 ug of fusion protein in 1 hour to 85 % completion at 30C in a buffer containing 50 mM Tris-HCl, pH 8.0, 0.5 mM EDTA, and 1 mM DTT.
TEV recognizes the amino acid sequence of the general form E-X-X-Y-X-Q (or S)/X’, and cleaves between Q (or S)/X’. In this form X and X’ stand for any of the amino acid residues, except that X’ cannot be P. The optimal cleavage site is ENLYFQ/G.
Purity
>90%, by SDS-PAGE
Predicted Molecular Mass
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
TEV recognizes the amino acid sequence of the general form E-X-X-Y-X-Q (or S)/X', and cleaves between Q (or S)/X'. In this form X and X' stand for any of the amino acid residues, except that X' cannot be P. The optimal cleavage site is ENLYFQ/G. As having the absolute specificity and wildly using conditions like broad pH range and ionic strength, the TEV protease became more versatile than EK, thrombin and other protease used in biochemical applications, especially recombinant protein production.
Application Notes
The optimal temperature for cleavage is 30C; however, the enzyme can be used at temperatures as low as 4C.
Protein / Peptide Type
Recombinant Protein
Formulation, Preparation and Storage
NBP2-61441
| Formulation | 0.2 um filtered solution in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 5 mM EDTA, 10 mM GSH |
| Preservative | 50% glycerol |
| Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20C. Avoid freeze-thaw cycles. |
Background: TEV Protease
Long Name
Tobacco Etch Virus Protease
Alternate Names
NIa
Gene Symbol
TEVgp1
Additional TEV Protease Products
Product Documents for Recombinant TEV Protease His (N-Term) Protein
Product Specific Notices for Recombinant TEV Protease His (N-Term) Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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