Recombinant Human Thymidine Kinase 2 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-98864

Novus Biologicals, part of Bio-Techne
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NBP1-98864-50ug
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Thymidine Kinase 2 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 34-265 of Human Thymidine Kinase 2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMVQRRA WPPDKEQEKE KKSVICVEGN IASGKTTCLE FFSNATDVEV LTEPVSKWRN VRGHNPLGLM YHDASRWGLT LQTYVQLTML DRHTRPQVSS VRLMERSIHS ARYIFVENLY RSGKMPEVDY VVLSEWFDWI LRNMDVSVDL IVYLRTNPET CYQRLKKRCR EEEKVIPLEY LEAIHHLHEE WLIKGSLFPM AAPVLVIEAD HHMERMLELF EQNRDRILTP ENRKHCP

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

30.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Thymidine Kinase 2 His Protein

SDS-PAGE: Recombinant Human Thymidine Kinase 2 His Protein [NBP1-98864]

SDS-PAGE: Recombinant Human Thymidine Kinase 2 His Protein [NBP1-98864]

SDS-Page: Recombinant Human Thymidine Kinase 2 Protein [NBP1-98864] - 15% SDS-PAGE (3ug)
Recombinant Human Thymidine Kinase 2 His Protein

SDS-PAGE: Recombinant Human Thymidine Kinase 2 Protein [NBP1-98864] -

SDS-PAGE: Recombinant Human Thymidine Kinase 2 Protein [NBP1-98864] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-98864
Formulation 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, 0.5M NaCl, 250mM Imidazole, 0.1mM PMSF
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Thymidine Kinase 2

TK2, also known as thymidine kinase 2 mitochondrial, belongs to the DCK/DGK family. Thymidine kinase is an enzyme, a phosphotransferase (a kinase): 2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase, It can be found in most living cells. It is present in two forms in mammalian cells, TK1 and TK2. Certain viruses also have genetic information for expression of viral thymidine kinases. Thymidine kinases have a key function in the synthesis of DNA and thereby in cell division, as they are part of the unique reaction chain to introduce deoxythymidine into the DNA. Defects in TK2 are a cause of mitochondrial DNA depletion syndrome type 2 (MTDPS2). A disorder characterized primarily by childhood onset of muscle weakness associated with depletion of mtDNA in skeletal muscle. Recombinant human TK2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

EC 2.7.1.21, MTDPS2, MTTK, Mt-TK, thymidine kinase 2, mitochondrial

Gene Symbol

TK2

Additional Thymidine Kinase 2 Products

Product Documents for Recombinant Human Thymidine Kinase 2 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Thymidine Kinase 2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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