Recombinant Human TIGAR/C12orf5 TAT Protein

Name: Recombinant Human TIGAR/C12orf5 TAT Protein
Brand: Novus Biologicals
SKU: NBP2-35063

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35063

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

Q9NQ88

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all TIGAR/C12orf5 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 283 amino acids corresponding to TIGAR/C12orf5 Source: E. coli

Uniprot ID: Q9NQ88

Amino Acid Sequence: MARFALTVVR HGETRFNKEK IIQGQGVDEP LSETGFKQAA AAGIFLNNVK FTHAFSSDLM RTKQTMHGIL ERSKFCKDMT VKYDSRLRER KYGVVEGKAL SELRAMAKAA REECPVFTPP GGETLDQVKM RGIDFFEFLC QLILKEADQK EQFSQGSPSN CLETSLAEIF PLGKNHSSKV NSDSGIPGLA ASVLVVSHGA YMRSLFDYFL TDLKCSLPAT LSRSELMSVT PNTGMSLFII NFEEGREVKP TVQCICMNLQ DHLNGLTETR GGYGRKKRRQ RRR

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of TIGAR/C12orf5 as determined by LAL method.

Predicted Molecular Mass

31.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

TIGAR protein is fully biologically active when compared to standard. The biological activity determined by its ability to protect U2OS cells from apoptosis induced by hydrogen peroxide is in a concentration range of 0.1-5.0 ug/ml, after pretreating with rHuTIGAR-TAT for 4 hours.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human TIGAR/C12orf5 TAT Protein

SDS-PAGE: Recombinant Human TIGAR/C12orf5 TAT Protein [NBP2-35063]

SDS-Page: Human TIGAR/C12orf5 TAT Protein [NBP2-35063]

Formulation, Preparation and Storage

NBP2-35063

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile, 0.1% TFA.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: TIGAR/C12orf5

The TP53-inducible glycolysis and apoptosis regulator (TIGAR), also named fructose-2, 6-bisphosphatase TIGAR, is an enzyme that in humans is encoded by the C12orf5 gene. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. TIGAR activity can have multiple cellular effects. Recombinant human TIGAR-TAT expressed in E. coli is a 31.7 kDa protein containing 283 amino-acid residues, including the 270 residues of fulllength TIGAR fused to a 13-residue C-terminal peptide containing the TAT transduction domain (GGYGRKKRRQRRR).

Long Name

TP53-induced Glycolysis and Apoptosis Regulator

Alternate Names

C12orf5

Gene Symbol

TIGAR

UniProt

Q9NQ88 (Human)

Additional TIGAR/C12orf5 Products

Product Documents for Recombinant Human TIGAR/C12orf5 TAT Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human TIGAR/C12orf5 TAT Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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