Recombinant Human Transgelin/TAGLN/SM22 alpha His Protein

Name: Recombinant Human Transgelin/TAGLN/SM22 alpha His Protein
Brand: Novus Biologicals
SKU: NBP1-45267

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45267

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Background
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Transgelin/TAGLN Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-201 of Human Transgelin/TAGLN/SM22 alpha

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MANKGPSYGM SREVQSKIEK KYDEELEERL VEWIIVQCGP DVGRPDRGRL GFQVWLKNGV ILSKLVNSLY PDGSKPVKVP ENPPSMVFKQ MEQVAQFLKA AEDYGVIKTD MFQTVDLFEG KDMAAVQRTL MALGSLAVTK NDGHYRGDPN WFMKKAQEHK REFTESQLQE GKHVIGLQMG SNRGASQAGM TGYGRPRQII S

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

24.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Transgelin/TAGLN/SM22 alpha His Protein

SDS-PAGE: Recombinant Human Transgelin/TAGLN/SM22 alpha His Protein [NBP1-45267]

SDS-Page: Recombinant Human Transgelin/TAGLN/SM22 alpha Protein [NBP1-45267] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-45267

Formulation	20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 1 mM DTT
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Transgelin/TAGLN

TAGLN is a transformation and shape-change sensitive actin cross-linking/gelling protein that belongs to the calponin family. This protein is expressed abundantly in fibroblasts and smooth muscle. It is involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence. During embryogenesis, TAGLN is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. It is downregulated in several transformed cell lines, indicating that a reduction of TAGLN expression may be an early indicator of the onset of transformation. Recombinant human TAGLN protein, fused to his-tag at N-terminus was expressed in E.coli and purified by using conventional chromatography techniques

Alternate Names

SM22 alpha, TAGLN, WS3-10

Gene Symbol

TAGLN

Additional Transgelin/TAGLN Products

Product Documents for Recombinant Human Transgelin/TAGLN/SM22 alpha His Protein

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COA

Product Specific Notices for Recombinant Human Transgelin/TAGLN/SM22 alpha His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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