Recombinant Human TRIP1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23031

Novus Biologicals, part of Bio-Techne
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NBP2-23031
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all TRIP1 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-325 of Human TRIP1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMKPILLQ GHERSITQIK YNREGDLLFT VAKDPIVNVW YSVNGERLGT YMGHTGAVWC VDADWDTKHV LTGSADNSCR LWDCETGKQL ALLKTNSAVR TCGFDFGGNI IMFSTDKQMG YQCFVSFFDL RDPSQIDNNE PYMKIPCNDS KITSAVWGPL GECIIAGHES GELNQYSAKS GEVLVNVKEH SRQINDIQLS RDMTMFVTAS KDNTAKLFDS TTLEHQKTFR TERPVNSAAL SPNYDHVVLG GGQEAMDVTT TSTRIGKFEA RFFHLAFEEE FGRVKGHFGP INSVAFHPDG KSYSSGGEDG YVRIHYFDPQ YFEFEFEA

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

38.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human TRIP1 His Protein

SDS-PAGE: Recombinant Human TRIP1 His Protein [NBP2-23031]

SDS-PAGE: Recombinant Human TRIP1 His Protein [NBP2-23031]

SDS-Page: TRIP1 Protein [NBP2-23031]

Formulation, Preparation and Storage

NBP2-23031
Formulation 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.4M UREA
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRIP1

EIF3I is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2: GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Recombinant human EIF3I protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

eIF-3-beta, eIF3-beta, eIF3i, eIF3-p36, EIF3S2TGF-beta receptor-interacting protein 1, Eukaryotic translation initiation factor 3 subunit 2, eukaryotic translation initiation factor 3 subunit I, eukaryotic translation initiation factor 3, subunit 2 (beta, 36kD), eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa, eukaryotic translation initiation factor 3, subunit I, predicted protein of HQ2242, TGFbeta receptor-interacting protein 1, TRIP-1, TRIP-1eIF3 p36, TRIP1PRO2242

Gene Symbol

EIF3I

Additional TRIP1 Products

Product Documents for Recombinant Human TRIP1 His Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human TRIP1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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