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Recombinant Human Troponin I type 2 (fast skeletal) His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22893

Novus Biologicals, part of Bio-Techne
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NBP2-22893

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-182 of Human Troponin I type 2 (fast skeletal)

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGDEEKRNRA ITARRQHLKS VMLQIAATEL EKEESRREAE KQNYLAEHCP PLHIPGSMSE VQELCKQLHA KIDAAEEEKY DMEVRVQKTS KELEDMNQKL FDLRGKFKRP PLRRVRMSAD AMLKALLGSK HKVCMDLRAN LKQVKKEDTE KERDLRDVGD WRKNIEEKSG MEGRKKMFES ES

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

23.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Troponin I type 2 (fast skeletal) His Protein

SDS-PAGE: Recombinant Human Troponin I type 2 (fast skeletal) His Protein [NBP2-22893]

SDS-PAGE: Recombinant Human Troponin I type 2 (fast skeletal) His Protein [NBP2-22893]

SDS-Page: Recombinant Human Troponin I type 2 (fast skeletal) Protein [NBP2-22893]

Formulation, Preparation and Storage

NBP2-22893
Formulation 20 mM Tris-HCl buffer (pH 8.0), 2M UREA, 20% glycerol, 0.2 M NaCl
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Troponin I type 2 (fast skeletal)

Troponin I, fast skeletal muscle, also known as TNNI2, is a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible for the calcium-dependent regulation of striated muscle contraction. This protein also suppresses tumor growth in human ovarian carcinoma. Mutations in TNNI2 cause myopathy and distal arthrogryposis type 2B. Recombinant human TNNI2 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

skeletal, fast, troponin I type 2 (skeletal, fast), troponin I, fast skeletal muscle, Troponin I, fast-twitch isoform, troponin I, fast-twitch skeletal muscle isoform

Gene Symbol

TNNI2

Additional Troponin I type 2 (fast skeletal) Products

Product Documents for Recombinant Human Troponin I type 2 (fast skeletal) His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human Troponin I type 2 (fast skeletal) His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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