Recombinant Human UBE2M/Ubc12 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35116

Novus Biologicals, part of Bio-Techne
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NBP2-35116-100ug
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Key Product Details

Source

E. coli

Accession #

P61081

Conjugate

Unconjugated

Applications

SDS-PAGE

View all UBE2M/Ubc12 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 183 amino acids corresponding to UBE2M/Ubc12 Source: E. coli

Uniprot ID: P61081

Amino Acid Sequence: MIKLFSLKQQ KKEEESAGGT KGSSKKASAA QLRIQKDINE LNLPKTCDIS FSDPDDLLNF KLVICPDEGF YKSGKFVFSF KVGQGYPHDP PKVKCETMVY HPNIDLEGNV CLNILREDWK PVLTINSIIY GLQYLFLEPN PEDPLNKEAA EVLQNNRRLF EQNVQRSMRG GYIGSTYFER CLK

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of UBE2M/Ubc12 as determined by LAL method.

Predicted Molecular Mass

20.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35116
Formulation A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 6.5, with 125 mM NaCl, 10 % Glycerol, 1 mM DTT.
Preservative 5% Trehalose
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Format Carrier-Free
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: UBE2M/Ubc12

Human Ubquitin Conjugating Enzyme E2 M (UBE2M)/UBC12 is is functional in in vitro NEDDylation reactions. It has been shown to form a thioester linkage with NEDD8 in the presence of the NEDD8 activating enzyme complex Uba3/APP-BP1. APPBP1 binds to the amyloid precursor protein (APP) carboxy terminal domain and is important in conjunction with Uba3 and UbcH12 in driving cells through the S to M checkpoint. It was demonstrated to be the E2 responsible for the NEDDylation of the Cul-1 component of the SCF (beta-TRCP) complex which is important as the E3-ligase in the ubiquitinylation of I-kappaB-alpha. NEDDylation of Cul-1 is essential for conjugation and processing of NF-kappaB p105 by SCF (beta-TRCP) following phosphorylation of the complex. A dominant negative form of UbcH12, previously demonstrated to sequester NEDD8 and inhibit its conjugation, inhibits both conjugation and processing of p105, which is alleviated by wild-type UbcH12.

Alternate Names

UBC-RS2, UBC12, UbcH12

Gene Symbol

UBE2M

UniProt

P61081 (Human)

Additional UBE2M/Ubc12 Products

Product Documents for Recombinant Human UBE2M/Ubc12 Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human UBE2M/Ubc12 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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