Recombinant Human VEGF 165 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07101

Novus Biologicals, part of Bio-Techne
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NBP3-07101-100ug
NBP3-07101-10ug
NBP3-07101-1mg
NBP3-07101-500ug
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Key Product Details

Source

Yeast

Conjugate

Unconjugated

Applications

Bioactivity

View all VEGF 165 Proteins and Enzymes »

Product Specifications

Description

A disulfide-linked homodimeric protein consisting of two 166 amino acid polypeptide chains corresponding to VEGF 165. As a result of glycosylation, it migrates to at least three bands with molecular weights ranging from 20-31 kDa in SDS-PAGE under reducing conditions Source: Yeast

Uniprot ID: P15692-4

Amino Acid Sequence: MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of VEGF 165 as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is between 1.0-8.0 ng/mL.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP3-07101
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS (pH 7.4), 0.02% Tween-20.
Preservative No Preservative
Concentration Lyoph
Reconstitution Briefly centrifuge prior to opening vial to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to 0.1-1.0 mg/mL.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: VEGF 165

VEGF (Vascular endothelial growth factor) is the only growth factor that stimulates vascular permeability. It promotes endothelial proliferation and survival, angiogenesis, vasculognesis and inhibits apoptosis. VEGF mitogenic activity is specific for endothelial cell and that makes it distinct among other growth factors. VEGF is thought to be important in the pathophysiology of neuronal and other tumors, by functioning as a promter of angiogenesis for human gliomas. Human VEGF occurs in several molecular variants and the 165 form is the most common form in most tissues. Biological activities of VEGF are not species-specific and glycosylation is not required for biological activity.

Alternate Names

MVCD1, vascular endothelial growth factor A, Vascular permeability factor, VEGF-A, VEGFMGC70609, VPFvascular endothelial growth factor, biosimilar antibody, biosimilars

Gene Symbol

VEGFA

Additional VEGF 165 Products

Product Documents for Recombinant Human VEGF 165 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human VEGF 165 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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