Recombinant Human VPS25 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-99072

Novus Biologicals, part of Bio-Techne
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NBP1-99072-0.5mg
NBP1-99072-100ug
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all VPS25 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-176 of Human VPS25

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAMSFE WPWQYRFPPF FTLQPNVDTR QKQLAAWCSL VLSFCRLHKQ SSMTVMEAQE SPLFNNVKLQ RKLPVESIQI VLEELRKKGN LEWLDKSKSS FLIMWRRPEE WGKLIYQWVS RSGQNNSVFT LYELTNGEDT EDEEFHGLDE ATLLRALQAL QQEHKAEIIT VSDGRGVKFF

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

23.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human VPS25 His Protein

SDS-PAGE: Recombinant Human VPS25 His Protein [NBP1-99072]

SDS-PAGE: Recombinant Human VPS25 His Protein [NBP1-99072]

SDS-Page: Recombinant Human VPS25 His Protein [NBP1-99072] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Formulation, Preparation and Storage

NBP1-99072
Formulation 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VPS25

VPS25, also known as vacuolar protein-sorting-associated protein 25, consists of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may play a role in transcription regulation, possibly via its interaction with ELL. Recombinant human VPS25 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

Dermal papilla-derived protein 9, DERP9, DERP9hVps25, EAP20, EAP20FAP20, ELL-associated protein of 20 kDa, ESCRT-II complex subunit VPS25, FAP20, MGC10540, vacuolar protein sorting 25 (yeast), vacuolar protein sorting 25 homolog (S. cerevisiae), vacuolar protein-sorting-associated protein 25

Gene Symbol

VPS25

Additional VPS25 Products

Product Documents for Recombinant Human VPS25 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human VPS25 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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