Recombinant Human Wnt-7a His Protein

Name: Recombinant Human Wnt-7a His Protein
Brand: Novus Biologicals
SKU: NBP2-22946

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22946

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Technical Data
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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Wnt-7a Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 32-349 of Human Wnt-7a

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSLGASIIC NKIPGLAPRQ RAICQSRPDA IIVIGEGSQM GLDECQFQFR NGRWNCSALG ERTVFGKELK VGSREAAFTY AIIAAGVAHA ITAACTQGNL SDCGCDKEKQ GQYHRDEGWK WGGCSADIRY GIGFAKVFVD AREIKQNART LMNLHNNEAG RKILEENMKL ECKCHGVSGS CTTKTCWTTL PQFRELGYVL KDKYNEAVHV EPVRASRNKR PTFLKIKKPL SYRKPMDTDL VYIEKSPNYC EEDPVTGSVG TQGRACNKTA PQASGCDLMC CGRGYNTHQY ARVWQCNCKF HWCCYVKCNT CSERTEMYTC K

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Wnt-7a His Protein

SDS-PAGE: Recombinant Human Wnt-7a His Protein [NBP2-22946]

SDS-Page: Recombinant Human Wnt-7a Protein [NBP2-22946]

Formulation, Preparation and Storage

NBP2-22946

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 0.4M UREA
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Wnt-7a

WNT7A is a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is involved in the development of the anterior-posterior axis in the female reproductive tract, and also plays a critical role in uterine smooth muscle pattering and maintenance of adult uterine function. Mutations in this gene are associated with Fuhrmann and Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndromes. Recombinant human WNT7A protein, fused to His-tag at N-terminus, was expressed in E.coli .

Long Name

Wingless-type MMTV Integration Site Family, Member 7a

Alternate Names

Wnt7a

Gene Symbol

WNT7A

Additional Wnt-7a Products

Product Documents for Recombinant Human Wnt-7a His Protein

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COA

Product Specific Notices for Recombinant Human Wnt-7a His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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