Goat anti-Monkey IgA/IgG/IgM Fc Secondary Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-69097
Key Product Details
Species Reactivity
Monkey
Applications
Dot Blot, ELISA, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Highly purified immunoglobulin fractions containing IgG, IgA and IgM isolated from monkey serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Reactivity Notes
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Precipitation reactions have been observed with immunoglobulins in Serum of other old-world Monkeys, including Cercopithecus, Cynomolgus and Baboon. The product may also react with other species as has been observed for Chimpanzee and man.
Specificity
The defined antibody specificity is directed to the major isotypes of the monkey immunoglobulin system, including antibody to common determinants and to class specific determinants as tested against as tested in immunoelectrophoresis and double radial immunodiffusion (Ouchterlony). In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal monkey plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained.
Clonality
Polyclonal
Host
Goat
Description
The lyophilized product is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Applications for Goat anti-Monkey IgA/IgG/IgM Fc Secondary Antibody
Application
Recommended Usage
Dot Blot
1:100-1:2000
ELISA
1:100-1:2000
Immunoblotting
1:100 - 1:2000
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Application Notes
Indirect immunofluorescence.
Formulation, Preparation, and Storage
Purification
Ion exchange chromatography
Reconstitution
Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.
Formulation
Lyophilized from PBS (pH 7.2)
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgA/IgG/IgM Fc
Additional IgA/IgG/IgM Fc Products
Product Documents for Goat anti-Monkey IgA/IgG/IgM Fc Secondary Antibody
Product Specific Notices for Goat anti-Monkey IgA/IgG/IgM Fc Secondary Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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