Goat anti-Human IgE Heavy Chain Secondary Antibody [Janelia Fluor 549] (Pre-adsorbed)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-74930JF549
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Janelia Fluor 549
Antibody Source
Polyclonal Goat IgG
Format
Pre-adsorbed
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary
Immunogen
Purified human IgE, (Epsilon chain)
Reactivity Notes
Based on IEP, this antibody reacts with heavy (epsilon) chains on human IgE.
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy (epsilon) chains on human IgE. Cross Adsorbed: This antibody has been pre-adsorbed against bovine, mouse, and rabbit serum proteins to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with bovine, mouse, rabbit serum proteins.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
ELISA
1:100-1:2000
Flow Cytometry
1:10-1:1000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50mM Sodium Borate
Format
Pre-adsorbed
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: IgE Heavy Chain
Alternate Names
Constant Region of Heavy Chain of IgE, Ig Epsilon Chain C Region, Ig Epsilon Chain C Region ND, IgE, Immunoglobulin Epsilon, immunoglobulin heavy constant epsilon
Additional IgE Heavy Chain Products
Product Specific Notices
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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